National Center for International Research on Animal Gut Nutrition, Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal Health, Laboratory of Gastrointestinal Microbiology, Joint International Research Laboratory of Animal Health and Food Safety, College of Animal Science and Technology , Nanjing Agricultural University , Nanjing 210095 , China.
J Agric Food Chem. 2019 Jan 16;67(2):578-588. doi: 10.1021/acs.jafc.8b05679. Epub 2019 Jan 3.
Suckling piglets were used to investigate the response of colonic mucosa-associated microbiota composition, mucosal immune homeostasis, and barrier function to early life galactooligosaccharides (GOS) intervention. Ten milliliter 1 g/kg body weight GOS solutions and physiological saline solutions were fed to the newborn piglets in the GOS group and in the control (CON) group a week time, respectively. Six piglets from each group were euthanized on day 8 and day 21. GOS piglets had a higher abundance of short-chain fatty acids (SCFAs) producer such as Prevotella, Barnesiella, Parabacteroides, and Unclassified Porphyromonadaceae in colonic mucosa ( P < 0.05). In addition, the total SCFAs level in colonic digesta of GOS piglets increased on day 8 ( P < 0.05) and day 21 ( P = 0.064). Meanwhile, a higher SCFAs concentration in colon of the GOS piglets altered the gene expression of inflammatory cytokines (IL-8 and IL-10) and barrier proteins (ZO-1 and Claudin-1) through regulating the phosphorylation of the NFκB and AMPK signaling pathway. In summary, these results provide important insights and understandings to reveal the relationship between the mucosal microbiota colonization and intestinal function at the early life stage of piglets.
我们使用哺乳仔猪来研究结肠黏膜相关微生物群落组成、黏膜免疫稳态和屏障功能对生命早期低聚半乳糖（GOS）干预的反应。GOS 组和对照组（CON）的新生仔猪分别在一周内用 10 毫升 1g/kg 体重的 GOS 溶液和生理盐水进行喂养。每组 6 头仔猪分别于第 8 天和第 21 天安乐死。GOS 仔猪的结肠黏膜中短链脂肪酸（SCFA）产生菌（如普雷沃氏菌、巴恩斯氏菌、拟杆菌和未分类的卟啉单胞菌科）丰度更高（P<0.05）。此外，GOS 仔猪结肠内容物中的总 SCFA 水平在第 8 天（P<0.05）和第 21 天（P=0.064）增加。同时，GOS 仔猪结肠中更高的 SCFA 浓度通过调节 NFκB 和 AMPK 信号通路的磷酸化，改变了炎症细胞因子（IL-8 和 IL-10）和屏障蛋白（ZO-1 和 Claudin-1）的基因表达。总之，这些结果为揭示仔猪生命早期黏膜微生物定殖与肠道功能之间的关系提供了重要的见解和认识。
