Sarkar V K, De U K, Kala A, Chauhan A, Verma A K, Paul B R, Soni S, Chaudhuri P, Patra M K, Gaur G K
Division of Medicine, ICAR-Indian Veterinary Research Institute, Izatnagar 243122 (UP), India.
Division of Animal Nutrition, ICAR-Indian Veterinary Research Institute, Izatnagar 243122 (UP), India.
Benef Microbes. 2023 Feb 1;14(3):197-208. doi: 10.3920/BM2022.0032.
The objective of this study was to examine the effects of early-life host specific probiotic and lactoferrin (LF) supplementations on diarrhoea incidence, iron (Fe)-zinc (Zn) balance and antioxidant capabilities in serum of neonatal piglets. A total of eight sow litters obtained from parity matched sows were randomly divided into four groups and assigned to one of the four interventions: control (2.0 ml normal saline), bovine lactoferrin (bLF) (100 mg bLF in normal saline), probiotic (Pb) (1×109 cfu of swine origin Pediococcus acidilactici FT28 strain) and bLF+Pb (both 100 mg bLF and 1×109 cfu of P. acidilactici FT28). All the piglets received supplementations once daily orally for first 7 days of life. The incidence of diarrhoea markedly decreased in bLF group compared to control group. Notably, no incidences of diarrhoea were recorded in Pb and bLF+Pb groups. The Zn and Fe concentrations were significantly increased from day 7 to 21 in bLF and on day 21 in bLF+Pb group. No such changes were noted in Pb group. Total antioxidant capacity (TAC) in serum was significantly increased on days 7 and 15 in bLF group and on days 7 and 21 in bLF+Pb group. Malonaldehyde concentration was markedly reduced from day 7 to 21 in bLF and bLF+Pb groups. The concentrations of nitrate on days 15 and 21 and malonaldehyde on day 7 were significantly higher in Pb group, but mean TAC was unaltered from day 0 to 21. Although no correlation between the incidence of diarrhoea and Zn/Fe and oxidant/antioxidant homeostasis was noted in the Pb group, the supplementation of P. acidilactici FT28 alone was sufficient to prevent the incidence of diarrhoea in neonatal piglets. Taken together, it is concluded that strategic supplementation of P. acidilactici FT28 in early life could help in preventing diarrhoea until weaning of piglets.
本研究的目的是检测早期特定宿主益生菌和乳铁蛋白（LF）补充剂对新生仔猪腹泻发病率、铁（Fe）-锌（Zn）平衡及血清抗氧化能力的影响。从经产情况匹配的母猪所产的八窝仔猪中随机选取，将其分为四组并分别给予四种干预措施之一：对照组（2.0毫升生理盐水）、牛乳铁蛋白（bLF）组（100毫克bLF溶于生理盐水）、益生菌（Pb）组（1×10⁹cfu猪源嗜酸乳杆菌FT28菌株）和bLF + Pb组（100毫克bLF和1×10⁹cfu嗜酸乳杆菌FT28）。所有仔猪在出生后的前7天每天口服一次补充剂。与对照组相比，bLF组腹泻发病率显著降低。值得注意的是，Pb组和bLF + Pb组未记录到腹泻病例。bLF组从第7天到第21天以及bLF + Pb组在第21天，锌和铁浓度显著升高。Pb组未观察到此类变化。bLF组在第7天和第15天以及bLF + Pb组在第7天和第21天血清总抗氧化能力（TAC）显著升高。bLF组和bLF + Pb组从第7天到第21天丙二醛浓度显著降低。Pb组在第15天和第21天的硝酸盐浓度以及第7天的丙二醛浓度显著更高，但从第0天到第21天平均TAC未改变。虽然在Pb组未观察到腹泻发病率与锌/铁及氧化/抗氧化稳态之间的相关性，但单独补充嗜酸乳杆菌FT28足以预防新生仔猪腹泻的发生。综上所述，得出结论：在生命早期战略性补充嗜酸乳杆菌FT28有助于预防仔猪断奶前的腹泻。
