State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
Genes (Basel). 2019 Jul 12;10(7):530. doi: 10.3390/genes10070530.
is a fairly diverse genus of bacteria with more than 260 species and subspecies. Many profiling methods have been developed to carry out phylogenetic analysis of this complex and diverse genus, but limitations remain since there is still a lack of comprehensive and accurate analytical method to profile this genus at species level. To overcome these limitations, a -specific primer set was developed targeting a hypervariable region in the gene-a single-copy gene that has undergone rapid mutation and evolution. The results showed that this methodology could accurately perform taxonomic identification of down to the species level. Its detection limit was as low as 10 colony-forming units (cfu)/mL for species. The assessment of detection specificity using the profiling method found that and genus could be distinguished, but non- Genus Complex could not be detected. The gene sequencing and Miseq high-throughput approach were adopted to estimate the richness and diversity of species in different ecosystems. The method was tested using kurut (fermented yak milk) samples and fecal samples of human, rat, and mouse. The results indicated that was the predominant gut species among Chinese, and accounted for the majority of lactobacilli in rat and mouse gut. Meanwhile, subsp. had the highest relative abundance of in kurut. Thus, this gene profiling method is expected to promote the application of for industrial production and therapeutic purpose.
是一个相当多样化的细菌属，拥有超过 260 个种和亚种。已经开发了许多分析方法来对这个复杂多样的属进行系统发育分析，但仍然存在局限性，因为仍然缺乏全面和准确的分析方法来对该属进行种水平的分析。为了克服这些局限性，开发了一种针对基因中一个高度可变区域的特异性引物对——一个单拷贝基因，该基因经历了快速的突变和进化。结果表明，该方法可以准确地对种水平的进行分类鉴定。其检测下限低至 10 个菌落形成单位(cfu)/mL。使用基因指纹图谱方法评估检测特异性发现，可以区分和属，但不能检测非属复合体。采用基因测序和 Miseq 高通量方法来估计不同生态系统中属的丰富度和多样性。该方法通过使用发酵牦牛乳（kurut）样本和人、鼠的粪便样本进行了测试。结果表明，是中国人肠道中主要的属，在大鼠和小鼠肠道中占主导地位的乳杆菌。同时，亚种在 kurut 中具有最高的相对丰度。因此，这种基因指纹图谱方法有望促进的应用，用于工业生产和治疗目的。
