Jia Hui-Ru, Geng Li-Li, Li Yun-He, Wang Qiang, Diao Qing-Yun, Zhou Ting, Dai Ping-Li
Ministry Key Laboratory of Pollinating Insect Biology, Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, China.
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Sci Rep. 2016 Apr 19;6:24664. doi: 10.1038/srep24664.
The honey bee has been regarded as a key species in the environmental risk assessment of biotech crops. Here, the potential adverse effects of Cry1Ie toxin on the midgut bacteria of the worker bees (Apis mellifera ligustica) were investigated under laboratory conditions. Newly emerged bees were fed with different concentrations of Cry1Ie toxin syrups (20 ng/mL, 200 ng/mL, and 20 μg/mL), pure sugar syrup, and 48 ppb of imidacloprid syrups, then sampled after 15 and 30 d. We characterized the dominant midgut bacteria and compared the composition and structure of the midgut bacterial community in all samples using the Illumina MiSeq platform targeting the V3-V4 regions of 16S rDNA. No significant differences in the diversity of the midgut bacteria were observed between the five treatments. This work was the first to show the effects of Cry1Ie toxin on honey bees, and our study provided a theoretical basis for the biosafety assessment of transgenic Cry1Ie maize.
蜜蜂被视为转基因作物环境风险评估中的关键物种。在此，我们在实验室条件下研究了Cry1Ie毒素对工蜂（意大利蜜蜂）中肠细菌的潜在不利影响。将新羽化的蜜蜂喂食不同浓度的Cry1Ie毒素糖浆（20纳克/毫升、200纳克/毫升和20微克/毫升）、纯糖浆以及48 ppb的吡虫啉糖浆，然后在15天和30天后进行采样。我们对优势中肠细菌进行了表征，并使用针对16S rDNA V3-V4区域的Illumina MiSeq平台比较了所有样本中中肠细菌群落的组成和结构。在这五种处理之间，未观察到中肠细菌多样性的显著差异。这项工作首次展示了Cry1Ie毒素对蜜蜂的影响，我们的研究为转基因Cry1Ie玉米的生物安全性评估提供了理论依据。
