Department of Stomatology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, People's Republic of China.
* Both the authors contributed equally to this article.
Cell Transplant. 2019 Nov;28(11):1432-1438. doi: 10.1177/0963689719861275. Epub 2019 Jul 24.
Chondral defects are challenging to repair because of the poor self-healing capacity of articular cartilage. The aim of this study was to compare and investigate the cartilage regeneration of stromal vascular fraction (SVF) cells and adipose-derived stem cells (ASCs) co-cultured with chondrocytes seeding on scaffolds composed of polyhydroxybutyrate (PHB)/poly-(hydroxybutyrate-co-hydroxyhexanoate) (PHBHHx). In this study, the cellular morphologies and proliferation capabilities on scaffolds were evaluated. Next, scaffolds with 1:1 co-culture of ASCs/SVF and chondrocytes were implanted into the full-thickness cartilage defects in rabbit knee for 10 weeks. Cells seeded on the scaffolds showed better adhesion, migration, and proliferation in vitro. Importantly, implantation with scaffolds with SVF and chondrocytes revealed more desirable in vivo healing outcomes. Our results illustrate a one-step surgical procedure for the regeneration of focal cartilage defects using a mixture of SVF from adipose tissue and uncultured chondrocytes.
软骨缺损的自我修复能力较差，因此很难修复。本研究旨在比较和研究间质血管成分 (SVF) 细胞与脂肪来源的干细胞 (ASCs) 与软骨细胞共培养后，在聚羟基丁酸酯 (PHB)/聚-(羟基丁酸-co-羟基己酸) (PHBHHx) 支架上的软骨再生情况。本研究评估了细胞在支架上的形态和增殖能力。接下来，将含有 1:1 共培养的 ASC/SVF 和软骨细胞的支架植入兔膝关节全层软骨缺损 10 周。结果显示，在支架上接种的细胞在体外具有更好的黏附、迁移和增殖能力。重要的是，植入 SVF 和软骨细胞的支架后，体内愈合效果更好。我们的研究结果表明，采用脂肪组织中的 SVF 与未培养的软骨细胞混合物，可以一步完成针对局灶性软骨缺损的再生手术。
