Institute of Medical Biology, Genetics and Clinical Genetics, Faculty of Medicine, Comenius University in Bratislava, 811 08 Bratislava, Slovak Republic.
Institute of Natural and Synthetic Polymers, Faculty of Chemical and Food Technology, Slovak University of Technology, 812 37 Bratislava, Slovak Republic.
Cell Transplant. 2021 Jan-Dec;30:9636897211021003. doi: 10.1177/09636897211021003.
Complex in vitro characterization of a blended material based on Poly(Lactic Acid), Poly(Hydroxybutyrate), and Thermoplastic Starch (PLA/PHB/TPS) was performed in order to evaluate its potential for application in the field of tissue engineering. We focused on the biological behavior of the material as well as its mechanical and morphological properties. We also focused on the potential of the blend to be processed by the 3D printer which would allow the fabrication of the custom-made scaffold. Several blends recipes were prepared and characterized. This material was then studied in the context of scaffold fabrication. Scaffold porosity, wettability, and cell-scaffold interaction were evaluated as well. MTT test and the direct contact cytotoxicity test were applied in order to evaluate the toxic potential of the blended material. Biocompatibility studies were performed on the human chondrocytes. According to our results, we assume that material had no toxic effect on the cell culture and therefore could be considered as biocompatible. Moreover, PLA/PHB/TPS blend is applicable for 3D printing. Printed scaffolds had highly porous morphology and were able to absorb water as well. In addition, cells could adhere and proliferate on the scaffold surface. We conclude that this blend has potential for scaffold engineering.
为了评估基于聚乳酸（PLA）、聚羟基丁酸酯（PHB）和热塑性淀粉（TPS）的混合材料在组织工程领域的应用潜力，我们对其进行了复杂的体外特性分析。我们重点研究了材料的生物行为及其机械和形态特性。我们还关注了该混合物通过 3D 打印机进行加工的潜力，这将允许制造定制支架。我们准备并表征了几种混合配方，然后在支架制造的背景下研究了该材料。评估了支架的孔隙率、润湿性和细胞-支架相互作用。还应用了 MTT 试验和直接接触细胞毒性试验来评估混合材料的潜在毒性。对人软骨细胞进行了生物相容性研究。根据我们的结果，我们假设该材料对细胞培养没有毒性作用，因此可以被认为是生物相容的。此外，PLA/PHB/TPS 混合物适用于 3D 打印。打印的支架具有高度多孔的形态，并且能够吸收水分。此外，细胞可以在支架表面附着和增殖。我们得出结论，该混合物具有支架工程的潜力。
