Department of Internal Medicine, Jinan Second People's Hospital, Jinan, China.
Int J Immunopathol Pharmacol. 2019 Jan-Dec;33:2058738419866379. doi: 10.1177/2058738419866379.
We aimed to analyze the action of berberine on the neuropathic pain and neuroglia activation in experimental diabetes mellitus (DM) model. Diabetes in mice was induced by intraperitoneal injection of streptozotocin (STZ) followed by the administration of berberine. Mechanical allodynia and thermal hyperalgesia and activations of microglia and astrocytes were evaluated. The levels of pro-inflammatory cytokines and protein expressions of inflammatory proteins were assessed by enzyme-linked immunosorbent assay (ELISA) and western blot, respectively. Our results revealed the anti-nociceptive effects of berberine in DM mice, supported by the improved mechanical threshold and thermal latency. In addition, berberine suppressed the activations of microglia and astrocytes in the spinal cords of diabetic mice. Berberine inhibited the expression of pro-inflammatory cytokines including tumor necrosis factor (TNF)-α, interleukin-6 (IL-6), and interleukin-1β (IL-1β), along with inflammatory proteins including iNOS and COX-2. Berberine suppressed neuropathic pain in STZ-induced diabetic mice, and this effect is related to the reduction on the neuroglia activation and inflammation associated with DM.
我们旨在分析小檗碱在实验性糖尿病（DM）模型中对神经病理性疼痛和神经胶质激活的作用。通过腹腔注射链脲佐菌素（STZ）诱导小鼠糖尿病，然后给予小檗碱。评估机械性痛觉过敏和热痛觉过敏以及小胶质细胞和星形胶质细胞的激活。通过酶联免疫吸附测定（ELISA）和 Western blot 分别评估促炎细胞因子的水平和炎症蛋白的蛋白表达。我们的结果表明小檗碱在 DM 小鼠中具有抗伤害感受作用，这得到了机械阈值和热潜伏期改善的支持。此外，小檗碱抑制了糖尿病小鼠脊髓中小胶质细胞和星形胶质细胞的激活。小檗碱抑制了包括肿瘤坏死因子（TNF）-α、白细胞介素 6（IL-6）和白细胞介素 1β（IL-1β）在内的促炎细胞因子的表达，以及包括诱导型一氧化氮合酶（iNOS）和环氧化酶 2（COX-2）在内的炎症蛋白。小檗碱抑制了 STZ 诱导的糖尿病小鼠的神经病理性疼痛，这种作用与减少与糖尿病相关的神经胶质激活和炎症有关。
