Department of Oral and Maxillofacial Surgery, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Dental Research Center, Research Institute of Dental Sciences, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran; Department of Tissue Engineering, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
J Craniomaxillofac Surg. 2019 Aug;47(8):1266-1273. doi: 10.1016/j.jcms.2019.03.030. Epub 2019 Apr 19.
Human amniotic membranes (HAMs), as a biological membrane with healing, osteogenic, and cell therapy potential, has been in the spotlight to enhance the outcomes of treating bone defects. Present study aims to clinically assess the potential of HAM loaded with buccal fat pad-derived stem cells (BFSCs) as an osteogenic coverage for onlay bone grafts to maxillomandibular bone defects.
Nine patients with jaw bone defects were enrolled in the present study. The patients were allocated to two study groups: Iliac crest bone graft with HAM coverage (n = 5), and Iliac bone grafts covered with HAM loaded with BFSCs (n = 4). Five months following the grafting and prior to implant placement, cone beam computed tomography was performed for radiomorphometric analysis.
The mean increase in bone width was found to be significantly greater in the HAM + BFSCs group (4.42 ± 1.03 mm versus 3.07 ± 0.73 mm, p < 0.05). Further, the changes in vertical dimension were greater in the HAM + BFSCs group (4.66 ± 1.06 mm versus 4.14 ± 1.03 mm, p > 0.05).
Combined use of HAM with mesenchymal stem cells may enhance bone regeneration specifically in the horizontal dimension. Moreover, this methodology reduces the amount of harvested autogenous bone and diminish secondary bone resorption.
人羊膜（HAM）作为一种具有愈合、成骨和细胞治疗潜力的生物膜，已成为增强治疗骨缺损效果的焦点。本研究旨在临床评估 HAM 负载颊脂垫来源干细胞（BFSCs）作为骨移植覆盖物治疗颌骨骨缺损的成骨潜力。
本研究纳入了 9 名颌骨缺损患者。将患者分为两组：带 HAM 覆盖的髂嵴骨移植（n=5）和带负载 BFSCs 的 HAM 覆盖的髂骨移植（n=4）。在移植后 5 个月，在植入物放置之前，进行锥形束计算机断层扫描进行放射形态计量学分析。
发现 HAM+BFSCs 组的骨宽度增加平均值明显大于 HAM 组（4.42±1.03mm 与 3.07±0.73mm，p<0.05）。此外，HAM+BFSCs 组的垂直维度变化更大（4.66±1.06mm 与 4.14±1.03mm，p>0.05）。
HAM 与间充质干细胞联合使用可能特别增强水平维度的骨再生。此外，这种方法减少了自体骨的采集量，并减少了继发性骨吸收。
