Yousefi Forouzan, Lavi Arab Fahimeh, Jaafari Mahmoud Reza, Rastin Maryam, Tabasi Nafiseh, Hatamipour Mahdi, Nikkhah Karim, Mahmoudi Mahmoud
Immunology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran.
EXCLI J. 2019 Jun 17;18:405-421. doi: 10.17179/excli2019-1366. eCollection 2019.
Curcuminoids are dietary complexes extracted from the seeds of . that contain curcumin, bisdemethoxycurcumin and desmethoxycurcumin. Curcuminoids are popular for their pleiotropic therapeutic functions, such as their anti-inflammatory and anti-oxidant effects. Nonetheless, their clinical use is associated with poor systemic bioavailability and insolubility. The nano-formulation of curcuminoids eliminates these shortcomings. In the present study, we explored immunoregulatory, proliferative and anti-oxidant effects of nanocurcuminoids on adipose-derived mesenchymal stem cells (AT-MSCs). Flow cytometry analysis and MTT assay were employed to explore the effects of nanocurcuminoids on the apoptosis and proliferation of adipose-derived MSCs (AT-MSCs). The anti-oxidant effect of nanocurcuminoids on AT-MSCs also was examined. The immune regulatory effect of nanocurcuminoids was evaluated by the flow cytometric measurement of the T regulatory (Treg) population. The expression of inflammatory and anti-inflammatory cytokines was quantified using real-time PCR. Our findings demonstrate that low concentrations of nanocurcuminoids are beneficial for MSC proliferation, protection of MSCs from apoptosis, reducing inflammatory cytokines and SOD activity. A high concentration of nanocurcuminoids increases the population of Tregs and elevates the expression of TGFβ and FOXP3 genes. The beneficial effects of nanocurcuminoids on AT-MSCs were mainly observed at low doses of nanocurcuminoids.
姜黄素类化合物是从……种子中提取的膳食复合物，包含姜黄素、双去甲氧基姜黄素和去甲氧基姜黄素。姜黄素类化合物因其多效性治疗功能而受到欢迎，例如它们的抗炎和抗氧化作用。尽管如此，它们的临床应用与较差的全身生物利用度和不溶性有关。姜黄素类化合物的纳米制剂消除了这些缺点。在本研究中，我们探讨了纳米姜黄素类化合物对脂肪来源间充质干细胞（AT-MSCs）的免疫调节、增殖和抗氧化作用。采用流式细胞术分析和MTT法来探究纳米姜黄素类化合物对脂肪来源间充质干细胞（AT-MSCs）凋亡和增殖的影响。还检测了纳米姜黄素类化合物对AT-MSCs的抗氧化作用。通过流式细胞术测量调节性T细胞（Treg）群体来评估纳米姜黄素类化合物的免疫调节作用。使用实时PCR定量炎症和抗炎细胞因子的表达。我们的研究结果表明，低浓度的纳米姜黄素类化合物有利于间充质干细胞增殖、保护间充质干细胞免于凋亡、减少炎症细胞因子和超氧化物歧化酶（SOD）活性。高浓度的纳米姜黄素类化合物增加了调节性T细胞的数量，并提高了转化生长因子β（TGFβ）和叉头框蛋白3（FOXP3）基因的表达。纳米姜黄素类化合物对AT-MSCs 的有益作用主要在低剂量的纳米姜黄素类化合物时观察到。
