Moradi-Gharibvand Nahid, Setayeshmehr Mohsen, Kazemi Mohammad, Safaee Azadeh, Khorsandi Laya Sadat, Nejad Darioush Bijan, Hasheminia Seyed Javad, Hashemibeni Batool
Department of Anatomical Sciences and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, I.R. Iran.
Department of Biomaterials, Nanotechnology, and Tissue Engineering, Faculty of Advanced Technologies in Medicine, Isfahan University of Medical Sciences, Isfahan, I.R. Iran.
Res Pharm Sci. 2022 Jul 14;17(4):372-382. doi: 10.4103/1735-5362.350238. eCollection 2022 Aug.
Pomegranate seed extract (PSE) possesses anticancer activities and healing effects. Adipose-derived stem cells (ADSCs) are being considered a new candidate for cancer treatment. The purpose of this study was to investigate the effect of PSE on the cell cycle and apoptosis of the MCF-7 cell line in the co-culture condition with ADSCs.
MCF-7 and ADSC cells (ratio 1/1) were cultured in a transwell plate with and without PSE (PSE-co-culture and co-culture groups). MCF-7 cells were cultured in monolayer without and with PSE (mono-culture and PSE-mono-culture groups). MCF-7 cell line was harvested on day 5 and cell viability, apoptotic activity, cell cycle, and gene expression were evaluated.
FINDINGS / RESULTS: The results of the MTT assay indicated that PSE at 100 μg/mL has the highest cytotoxicity on the MCF-7 in the PSE-co-culture group. The cell cycle analysis revealed that ADSCs in combination with PSE significantly increased the population of MCF-7 cells in the G1 phase, resulting in the arrest of MCF-7 cells cycle in the G0/G1 transition. In addition, the most apoptotic MCF-7 cells (41.5%) were detected in the same group. Expression of BAX and caspase3 genes were upregulated while anti-apoptotic (BCL-2) and angiogenesis inducer (VEGF) genes were downregulated in the PSE-co-culture group compared with the other groups.
ADSCs reduced cell viability and proliferation of MCF-7 cells in co-culture conditions and adding PSE to the medium increased the apoptosis of cancer cells. This study suggests that ADSCs with PSE can suppress tumor cells.
石榴籽提取物（PSE）具有抗癌活性和治疗作用。脂肪来源干细胞（ADSCs）正被视为癌症治疗的新候选者。本研究的目的是探讨PSE在与ADSCs共培养条件下对MCF-7细胞系细胞周期和凋亡的影响。
将MCF-7细胞和ADSC细胞（比例为1/1）在有和无PSE的Transwell板中培养（PSE共培养组和共培养组）。MCF-7细胞在有无PSE的情况下进行单层培养（单培养组和PSE单培养组）。在第5天收获MCF-7细胞系，并评估细胞活力、凋亡活性、细胞周期和基因表达。
MTT分析结果表明，在PSE共培养组中，100μg/mL的PSE对MCF-7细胞具有最高的细胞毒性。细胞周期分析显示，ADSCs与PSE联合使用显著增加了G1期MCF-7细胞的数量，导致MCF-7细胞周期在G0/G1转换期停滞。此外，在同一组中检测到最多的凋亡MCF-7细胞（41.5%）。与其他组相比，PSE共培养组中促凋亡基因BAX和caspase3的表达上调，而抗凋亡基因（BCL-2）和血管生成诱导因子（VEGF）基因的表达下调。
在共培养条件下，ADSCs降低了MCF-7细胞的活力和增殖，向培养基中添加PSE增加了癌细胞的凋亡。本研究表明，ADSCs与PSE联合可抑制肿瘤细胞。
