Mou Shaofeng, Zhou Zhongxin, He Yukai, Liu Fuxing, Gong Lili
Department of Otolaryngology, Liaocheng City People's Hospital, Liaocheng, Shandong 252000, P.R. China.
Department of Infection, Liaocheng City People's Hospital, Liaocheng, Shandong 252000, P.R. China.
Oncol Lett. 2017 Oct;14(4):4937-4942. doi: 10.3892/ol.2017.6739. Epub 2017 Aug 10.
Curcumin is a natural compound extracted from the dried rhizomes of (curcuma root or zedoary) that exhibits extensive pharmacological effects and low toxicity. The aim of the present study was to investigate whether curcumin inhibits cell proliferation and promotes apoptosis of laryngeal cancer through Bcl-2 and phosphoinositide 3-kinase (PI3K)/protein kinase B (Akt), and by upregulating microRNA-15a (miR-15a). It was demonstrated that curcumin inhibits cell proliferation, and promotes apoptosis and increased caspase-3 activity of human laryngeal cancer cells. Furthermore, curcumin decreased Bcl-2 and PI3K protein expression, and decreased the phospho (p)-Akt protein expression of human laryngeal cancer cells. Furthermore, curcumin activated miR-15a expression by human laryngeal cancer cells. Suppression of miR-15a expression reversed the anticancer effect of curcumin on cell proliferation of human laryngeal cancer cells and increased Bcl-2 and PI3K/Akt protein expression in AMC-HN-8 cells treated with 40 µM of curcumin. The results of the present study suggest that curcumin inhibits cell proliferation and promotes apoptosis of laryngeal cancer cells through Bcl-2 and PI3K/Akt, and by upregulating miR-15a.
姜黄素是从姜黄（姜根或莪术）干燥根茎中提取的一种天然化合物，具有广泛的药理作用且毒性较低。本研究的目的是探讨姜黄素是否通过Bcl-2和磷酸肌醇3激酶（PI3K）/蛋白激酶B（Akt），并上调微小RNA-15a（miR-15a）来抑制喉癌细胞增殖并促进其凋亡。结果表明，姜黄素可抑制人喉癌细胞的增殖，促进其凋亡并增加半胱天冬酶-3的活性。此外，姜黄素可降低人喉癌细胞中Bcl-2和PI3K蛋白的表达，并降低磷酸化（p）-Akt蛋白的表达。此外，姜黄素可激活人喉癌细胞中miR-15a的表达。抑制miR-15a的表达可逆转姜黄素对人喉癌细胞增殖的抗癌作用，并增加用40μM姜黄素处理的AMC-HN-8细胞中Bcl-2和PI3K/Akt蛋白的表达。本研究结果表明，姜黄素通过Bcl-2和PI3K/Akt，并上调miR-15a来抑制喉癌细胞增殖并促进其凋亡。
