Khodaverdi Majid, Razmi Gholamreza
Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.
Vet Res Forum. 2019 Spring;10(2):119-123. doi: 10.30466/vrf.2019.71293.1975. Epub 2019 Jun 15.
The aim of the present study was to determine seroprevalence of infection in stray cats and correlation with oocyst shedding and IFN-γ concentration. From April to August 2016, one hundred fifty-nine stray cats were captured from various localities in Mashhad area. The blood and fecal samples were collected from each cat. The serum samples were examined to detect antibodies against infection by ELISA assay and the fecal samples were microscopically examined for oocyst detection. The concentration changes of IFN-γ in serum samples of seropositive and seronegative cats were measured using ELISA kit. The results showed that 59.12% (94/159) of cats had antibodies against infection. The seroprevalence of infection in the adult cats above three years olds was higher than other groups. Regarding gender, month and region factors, the difference of seroprevalence of infection was not significant. In this study, the like oocyst (THLO) were detected in 2.56% (4/156) in fecal samples of one seropositive and three seronegative cats. Results also showed that the mean value for IFN-γ concentration in the seropositive cats was significantly higher than that of the seronegative cats. Based on the results, the high percentages of stray cats were infected with in this area. The IFN-γ concentration of seropositive cats was higher than that of the seronegative cats.
本研究的目的是确定流浪猫感染的血清阳性率以及与卵囊排出和干扰素-γ浓度的相关性。2016年4月至8月，从马什哈德地区的不同地点捕获了159只流浪猫。从每只猫采集血液和粪便样本。通过ELISA检测血清样本中抗感染的抗体，并通过显微镜检查粪便样本以检测卵囊。使用ELISA试剂盒测量血清阳性和血清阴性猫血清样本中干扰素-γ的浓度变化。结果显示，59.12%（94/159）的猫具有抗感染抗体。三岁以上成年猫的感染血清阳性率高于其他组。在性别、月份和地区因素方面，感染血清阳性率的差异不显著。在本研究中，在一只血清阳性猫和三只血清阴性猫的粪便样本中，2.56%（4/156）检测到类卵囊（THLO）。结果还显示，血清阳性猫的干扰素-γ浓度平均值显著高于血清阴性猫。基于这些结果，该地区流浪猫感染的比例很高。血清阳性猫的干扰素-γ浓度高于血清阴性猫。
