Dubey J P, Darrington C, Tiao N, Ferreira L R, Choudhary S, Molla B, Saville W J A, Tilahun G, Kwok O C H, Gebreyes W A
U.S. Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Building 1001, Beltsville, Maryland 20705-2350, USA.
J Parasitol. 2013 Feb;99(1):56-8. doi: 10.1645/GE-3229.1. Epub 2012 Jun 27.
Cats are important in the epidemiology of Toxoplasma gondii because they are the only hosts that excrete environmentally resistant oocysts in feces. In the present study, hearts, serum, and feces from 36 feral cats from Addis Ababa area, Ethiopia, were examined for T. gondii infection. Antibodies to T. gondii were determined with the modified agglutination test (MAT, cutoff 1:25); 33 cats were seropositive. Hearts of all 36 cats were homogenized, digested in pepsin, and bioassayed in mice. Feces were examined for T. gondii oocysts by bioassay in mice. Viable T. gondii was isolated from heart of 26 by bioassay in mice and from 25 seropositive and 1 seronegative cats. Toxoplasma gondii was isolated from feces (oocysts) by bioassay in mice. In total, viable T. gondii was isolated from 27 of the 36 cats, and these isolates were designated TgCatEt1 to TgCatEt27. The high prevalence of T. gondii oocysts in feces of 8 (19.4%) of 36 cats is of high epidemiologic significance. This is the first report of isolation of viable T. gondii from any host in Ethiopia.
猫在刚地弓形虫的流行病学中很重要，因为它们是唯一会在粪便中排出具有环境抵抗力的卵囊的宿主。在本研究中，对来自埃塞俄比亚亚的斯亚贝巴地区的36只野猫的心脏、血清和粪便进行了刚地弓形虫感染检测。采用改良凝集试验（MAT，截断值1:25）测定针对刚地弓形虫的抗体；33只猫血清呈阳性。将所有36只猫的心脏匀浆，用胃蛋白酶消化，并在小鼠中进行生物测定。通过在小鼠中进行生物测定来检测粪便中的刚地弓形虫卵囊。通过在小鼠中进行生物测定，从26只猫的心脏以及25只血清阳性和1只血清阴性的猫中分离出了活的刚地弓形虫。通过在小鼠中进行生物测定，从粪便（卵囊）中分离出了刚地弓形虫。总共从36只猫中的27只分离出了活的刚地弓形虫，这些分离株被命名为TgCatEt1至TgCatEt27。36只猫中有8只（19.4%）的粪便中刚地弓形虫卵囊的高流行率具有很高的流行病学意义。这是在埃塞俄比亚首次从任何宿主中分离出活的刚地弓形虫的报告。
