Zarei Leila, Mahdavi Rad Saied, Abdollahzade Fard Amin
Department of Anatomical Sciences, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran.
Student Research Committee, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran.
Vet Res Forum. 2019 Spring;10(2):133-138. doi: 10.30466/vrf.2019.74079.1996. Epub 2019 Jun 15.
Obesity causes many problems such as cardiovascular and chronic kidney diseases. The aim of this study was to evaluate the efficacy of retinoic acid and atorvastatin co-administration in kidneys protection against high-fat diet induced damage. Twenty-five male Wistar rats (200.00 ± 20.00 g) were divided into five groups: 1) Control (standard diet), 2) High-fat diet (cholesterol 1.00%, 75 days), 3) High-fat diet + atorvastatin (20.00 mg kg per day, orally, on the 30 day, for 45 consecutive days), 4) High-fat diet + retinoic acid (5 mg kg per day, orally, on the 30 day, for 45 consecutive days), and 5) High fat diet + atorvastatin and retinoic acid. At the end, blood and tissue samples were collected for biochemical and histological analyses. The results showed that atorvastatin and retinoic acid alone and in combination decreased cholesterol and low-density lipoprotein and increased high-density lipoprotein in high-fat diet. Also, atorvastatin - caused total antioxidant capacity increase and protein carbonyl content decrease the in the renal tissue. Atorvastatin also prevented high-fat diet-induced renal histological injury. Treatment with atorvastatin significantly mitigates high-fat diet-induced renal changes probably due to its potent antioxidant and lipid-lowering effects. The effect of retinoic acid in renal protection in a high-fat diet is far less than that of atorvastatin. The protective effect of the combination of these two agents in the high-fat diet on the kidneys seems to be due to the effect of atorvastatin.
肥胖会引发诸多问题，如心血管疾病和慢性肾病。本研究旨在评估维甲酸与阿托伐他汀联合用药对高脂饮食诱导的肾脏损伤的保护效果。将25只雄性Wistar大鼠（体重200.00 ± 20.00克）分为五组：1）对照组（标准饮食）；2）高脂饮食组（胆固醇1.00%，持续75天）；3）高脂饮食 + 阿托伐他汀组（每天口服20.00毫克/千克，第30天开始，连续45天）；4）高脂饮食 + 维甲酸组（每天口服5毫克/千克，第30天开始，连续45天）；5）高脂饮食 + 阿托伐他汀和维甲酸组。实验结束时，采集血液和组织样本进行生化和组织学分析。结果显示，单独使用阿托伐他汀和维甲酸以及二者联合使用均可降低高脂饮食组大鼠的胆固醇和低密度脂蛋白水平，并提高高密度脂蛋白水平。此外，阿托伐他汀可提高肾脏组织的总抗氧化能力并降低蛋白质羰基含量。阿托伐他汀还可预防高脂饮食诱导的肾脏组织学损伤。阿托伐他汀治疗可显著减轻高脂饮食诱导的肾脏变化，这可能归因于其强大的抗氧化和降脂作用。维甲酸对高脂饮食诱导的肾脏保护作用远不及阿托伐他汀。这两种药物联合使用对高脂饮食大鼠肾脏的保护作用似乎归因于阿托伐他汀的作用。
