Salahshoor Mohammad Reza, Mirzaei Fatemeh, Roshankhah Shiva, Jalili Parnian, Jalili Cyrus
Department of Anatomical Sciences, Medical School, Kermanshah University of Medical Sciences, Kermanshah, Iran.
Department of Anatomical Sciences, Medical School, Hamedan University of Medical Sciences, Hamedan, Iran.
Anat Cell Biol. 2019 Jun;52(2):183-190. doi: 10.5115/acb.2019.52.2.183. Epub 2019 Jun 30.
Nicotine is the most toxic factor of tobacco. Genistein is a phytoestrogen and antioxidant that has numerous health benefits. The aim of this study is to evaluate the effects of genistein against toxic properties of nicotine to the pancreas of mice. For this purpose, 48 male mice were randomly assigned into six groups (n=8): normal control, nicotine control (2.5 mg/kg), genistein (25 and 50 mg/kg), and nicotine+genistein (25 and 50 mg/kg) treated groups. Various doses of genistein and genistein+nicotine were administered intraperitoneally to animals for 4 weeks. The weight of pancreas, total antioxidant capacity and nitrite oxide of serum, insulin levels, and the number and diameter of islets of Langerhans were investigated. Nicotine administration reduced significantly total antioxidant capacity, insulin, pancreas weight, and the number and diameter of islets of Langerhans and increased nitrite oxide in serum compared to the control normal group (<0.05). Conversely, genistein and genistein+nicotine increased significantly insulin, total antioxidant capacity, and the number and diameter islets of Langerhans and decreased serum nitrite oxide compared to the nicotine control group. It seems that the genistein can improve pancreas damage following the nicotine administration.
尼古丁是烟草中最具毒性的因素。染料木黄酮是一种植物雌激素和抗氧化剂，对健康有诸多益处。本研究的目的是评估染料木黄酮对尼古丁对小鼠胰腺毒性作用的影响。为此，将48只雄性小鼠随机分为六组（n = 8）：正常对照组、尼古丁对照组（2.5毫克/千克）、染料木黄酮组（25毫克/千克和50毫克/千克）以及尼古丁+染料木黄酮组（25毫克/千克和50毫克/千克）处理组。将不同剂量的染料木黄酮和染料木黄酮+尼古丁腹腔注射给动物，持续4周。研究了胰腺重量、血清总抗氧化能力和一氧化氮、胰岛素水平以及胰岛的数量和直径。与正常对照组相比，给予尼古丁显著降低了血清总抗氧化能力、胰岛素、胰腺重量以及胰岛的数量和直径，并增加了血清一氧化氮水平（<0.05）。相反，与尼古丁对照组相比，染料木黄酮和染料木黄酮+尼古丁显著增加了胰岛素、总抗氧化能力以及胰岛的数量和直径，并降低了血清一氧化氮水平。似乎染料木黄酮可以改善尼古丁给药后对胰腺的损伤。
