Antidiabetic Activity of an Ayurvedic Formulation Chaturmukha Rasa: A Mechanism Based Study.

OBJECTIVES

The objective of this study was to evaluate antidiabetic activity of based on streptozotocin induced diabetes model, alpha amylase inhibitory activity, alpha Glucosidase inhibitory activity and inhibition of sucrase.

METHODS

was prepared as per Ayurvedic formulary. Antidiabetic activity was measured in experimentally streptozotocin induced rats. The dose was taken as 45 mg/kg, i.p. The antidiabetic activity of was compared Triphala Kwatha, a marketed formulation. Further In vitro ά-Amylase Inhibitory Assay, In vitro salivary amylase Inhibitory Assay, In vitro α-Glucosidase Inhibitory Assay and In vitro Sucrase Inhibitory Assay was performed with respect to . The IC50 value was calculated for all the above activity.

RESULTS

Streptozotocin with Acarbose showed significant decrease in blood glucose level whereas streptozotocin with Triphala kwatha showed more decrease in blood glucose level than Streptozotocin with Acarbose. The combination of Streptozotocin + Triphala kwatha + showed a significant decrease in blood glucose level on 21st day. In vitro ά-Amylase Inhibitory Assay the showed IC50 value 495.94 μl when compared with Acarbose 427.33 μl, respectively. In the α-Glucosidase Inhibitory Assay showed IC50 value 70.93 μl when compared with Acarbose 102.28 μl, respectively. In vitro Sucrase Inhibitory Assay showed IC50 value 415.4 μl when compared with Acarbose 371.43 μl, respectively.

CONCLUSION

This study supports that may inhibit diabetes by inhibition of salivary amylase or alpha Glucosidase or sucrase. This may be the mechanism by which inhibits diabetes. Further this study supports the usage of for the management of diabetes.