Liu Hyunju, Lee Jung In, Ahn Tae-Gyu
Department of Obstetrics and Gynecology, College of Medicine, Chosun University, Gwangju, Korea.
Department of Internal Medicine, College of Medicine, Chosun University, Gwangju, Korea.
Obstet Gynecol Sci. 2019 Jul;62(4):242-248. doi: 10.5468/ogs.2019.62.4.242. Epub 2019 Jun 19.
The purpose of this study was to determine the effect of quercetin on the antitumor activity of cisplatin and its side-effects.
EMT6 cells, a mouse breast cancer cell line, were injected subcutaneously in mice to generate a breast tumor-bearing mouse model. Experimental groups were divided into four groups: control (C), quercetin (Q), cisplatin (CP), and cisplatin+quercetin (CP+Q).
The tumor volume of the CP+Q group was significantly lower than that of the CP group. Serum blood urea nitrogen and creatinine levels in the CP+Q group were lower than those in the CP group. Renal γ-glutamyltranspeptidase and alkaline phosphatase activities were significantly higher in the CP+Q group than in the CP group, and the content of renal thiobarbituric acid reactive substance was significantly lower in the CP+Q group than that in the CP group. These results suggested that quercetin and cisplatin synergistically increased cellular toxicity in breast cancer cells and mediated cancer growth inhibition, thereby enhancing the antitumor effect of cisplatin compared to when only cisplatin was administered. Quercetin also reduced renal toxicity, which arose as a potential a side effect of cisplatin.
The enhanced antitumor effect of cisplatin and decreased renal toxicity after quercetin treatment suggested the applicability of quercetin as an adjuvant for chemotherapeutic agents.
本研究旨在确定槲皮素对顺铂抗肿瘤活性及其副作用的影响。
将小鼠乳腺癌细胞系EMT6细胞皮下注射到小鼠体内，建立荷瘤小鼠模型。实验组分为四组：对照组（C）、槲皮素组（Q）、顺铂组（CP）和顺铂+槲皮素组（CP+Q）。
CP+Q组的肿瘤体积显著低于CP组。CP+Q组血清尿素氮和肌酐水平低于CP组。CP+Q组肾γ-谷氨酰转肽酶和碱性磷酸酶活性显著高于CP组，CP+Q组肾硫代巴比妥酸反应物质含量显著低于CP组。这些结果表明，槲皮素和顺铂协同增加乳腺癌细胞的细胞毒性并介导癌症生长抑制，因此与仅给予顺铂相比，增强了顺铂的抗肿瘤作用。槲皮素还降低了作为顺铂潜在副作用出现的肾毒性。
槲皮素处理后顺铂抗肿瘤作用增强且肾毒性降低，表明槲皮素作为化疗药物佐剂的适用性。
