4-二甲基氨基偶氮苯干扰核糖体与内质网膜的结合。

4-Dimethylaminoazobenzene interferes with the binding of ribosomes to reticular membranes.

作者信息

Gupta R, Dani H M

机构信息

Department of Biochemistry, Panjab University, Chandigarh, India.

出版信息

Toxicol Lett. 1988 Jul;42(1):73-8. doi: 10.1016/0378-4274(88)90104-x.

DOI:10.1016/0378-4274(88)90104-x

PMID:3133839

Abstract

Treatment of chemically degranulated rough endoplasmic reticulum (RER) with 4-dimethylamino-azobenzene (4-DAB) partly damages binding of fresh polyribosomes to both the lighter and heavier fractions of reconstituted RER. Also, treatment of ribosomes with 4-DAB seemed to inhibit their binding to the chemically stripped RER (DRER) appearing both as lighter and heavier rough membrane layers after reconstitution. The interaction of 4-DAB with stripped microsomal fraction and ribosomes might be introducing certain conformational constraints into their proteins, thus crippling the binding of the ribosomes to reticular membranes.

摘要

用4-二甲基氨基偶氮苯（4-DAB）处理化学脱颗粒的粗面内质网（RER），会部分破坏新鲜多核糖体与重构RER较轻和较重部分的结合。此外，用4-DAB处理核糖体似乎会抑制它们与化学剥离的RER（DRER）的结合，DRER在重构后呈现为较轻和较重的粗面膜层。4-DAB与剥离的微粒体部分和核糖体的相互作用可能会给它们的蛋白质引入某些构象限制，从而削弱核糖体与网状膜的结合。