锐钛矿型（TiO₂）纳米颗粒的毒性评估：关于Medik应激反应改变和DNA损伤研究的初步研究

Khan Zeba, Shahwar Durre, Yunus Ansari Mohd Khalil, Chandel Rahul

Dept. of Botany, Aligarh Muslim University, Aligarh, India.

Dept of Biotechnology, Amity University, Haryana, India.

Heliyon. 2019 Jul 13;5(7):e02069. doi: 10.1016/j.heliyon.2019.e02069. eCollection 2019 Jul.

The research was targeted to investigate the effect of nano-TiO (anatase) on germination, vigour index, stress enzymes and mitotic cell cycle profile in lentils ( Medik.). Seed germination results indicated that TiO NP (Nanoparticle) at lowest concentration promotes seed germination, vigour index and biomass; however, at higher concentrations, they showed significant reduction in growth parameters and photosynthetic pigments in concentration-dependent manner. NP treatments triggered an excessive formation of reactive oxygen species (ROS) which was evident from increased production of stress enzymes, lipid peroxidation, augmented DNA damage and aberrant mitotic cell division. The results exhibit a dose-dependent modification of NP- mediated oxidative stress and genotoxicity in lentil.

该研究旨在调查纳米二氧化钛（锐钛矿型）对小扁豆（兵豆属）种子萌发、活力指数、应激酶和有丝分裂细胞周期谱的影响。种子萌发结果表明，最低浓度的二氧化钛纳米颗粒可促进种子萌发、活力指数和生物量；然而，在较高浓度下，它们会以浓度依赖的方式显著降低生长参数和光合色素。纳米颗粒处理引发了活性氧（ROS）的过度形成，这从应激酶产量增加、脂质过氧化、DNA损伤加剧和有丝分裂细胞分裂异常中明显可见。结果显示了纳米颗粒介导的氧化应激和遗传毒性在小扁豆中的剂量依赖性变化。