School of Chemistry, Biology and Material Engineering, Suzhou University of Science and Technology, Suzhou, China.
Suzhou Key Laboratory for Medical Biotechnology, Suzhou Vocational Health College, Suzhou, China.
J Cell Biochem. 2019 Nov;120(11):18600-18607. doi: 10.1002/jcb.28696. Epub 2019 Jul 24.
Lung cancer (especially, non-small cell lung cancer [NSCLC]) is one of the most malignant cancers in the world. Hinesol is the major component of the essential oil of Atractylodes lancea (Thunb.) DC and possesses the most promising anticancer function. However, the effects and molecular mechanism of hinesol on antiproliferation in NSCLC cells has not been well understood. In this study, we found that hinesol effectively inhibited the A549 and NCI-H1299 cells in a dose- and time-dependent manner by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide assay. In addition, hinesol induced cell cycle arrest at G0/G1 phase and apoptosis assessed by flow cytometry in A549 cells. Furthermore, Western blot analysis showed that hinesol decreased phosphorylation of mitogen-activated protein kinase, extracellular signal-regulated kinase, IκBα, and p65 inhibited the expressions of Bcl-2, cyclin D1 and upregulated the expression of Bax. Based on these results, hinesol might be a potential drug candidate of anti-NSCLC for therapy.
肺癌（特别是非小细胞肺癌[NSCLC]）是世界上最恶性的癌症之一。苍术素是苍术（白术）挥发油的主要成分，具有最有前途的抗癌功能。然而，苍术素对非小细胞肺癌细胞增殖的抑制作用及其分子机制尚不清楚。在这项研究中，我们发现苍术素通过 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐测定法以剂量和时间依赖的方式有效抑制 A549 和 NCI-H1299 细胞。此外，苍术素通过流式细胞术诱导 A549 细胞的细胞周期停滞在 G0/G1 期和凋亡。此外，Western blot 分析表明苍术素降低丝裂原活化蛋白激酶、细胞外信号调节激酶、IκBα 和 p65 的磷酸化，抑制 Bcl-2、细胞周期蛋白 D1 的表达，上调 Bax 的表达。基于这些结果，苍术素可能是一种有潜力的抗 NSCLC 治疗药物候选物。
