College of Fisheries, Henan Normal University, Xinxiang, 453007, PR China.
Synthetic Biology Engineering Lab of Henan Province, College of Life Sciences and Technology, Xinxiang Medical University, Xinxiang, 453003, PR China.
Int J Syst Evol Microbiol. 2019 Oct;69(10):3116-3120. doi: 10.1099/ijsem.0.003600.
A novel actinomycete strain, designated CFH S0054, was isolated from a soil sample collected from Xiaokongshan in Tengchong, Yunnan Province, in south-west PR China. Phylogenetic analysis based on its 16S rRNA gene sequence indicated that strain CFH S0054 belonged to the genus . Based on the results of 16S rRNA gene sequence analysis, strain CFH S0054 was most closely related to IFM 0354 (98.3 %), NBRC 100128 (97.9 %), NBRC 14405 (97.9 %) and CFH S0067 (97.8 %). Strain CFH S0054 contained C, C ω9, C 10-methyl (TBSA) and summed feature 3 as major cellular fatty acids and MK-8 (H4ω-cycl) as the major respiratory quinone. The polar lipid profile included phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside and unknown phospholipids. The cell wall contained -diaminopimelic acid, and the whole-cell hydrolysates were arabinose, galactose, glucose, fructose, ribose and mannose. The genome of strain CFH S0054 was 7.9 Mbp with a G+C content of 68.4 mol%, the average nucleotide identity (ANI) values between strain CFH S0054 and IFM0354 were low (ANIm<87.7 % and ANIb=84.1 %). On the basis of phylogenetic, physiological and chemotaxonomic data, strain CFHS0054 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed, The type strain is CFH S0054 (DSM 46763=JCM 30082).
一株新的放线菌菌株，命名为 CFH S0054，从中国西南部云南省腾冲县小空山的土壤样本中分离得到。基于 16S rRNA 基因序列的系统发育分析表明，菌株 CFH S0054 属于 属。基于 16S rRNA 基因序列分析的结果，菌株 CFH S0054 与 IFM 0354（98.3%）、NBRC 100128（97.9%）、NBRC 14405（97.9%）和 CFH S0067（97.8%）最为密切相关。菌株 CFH S0054 含有 C、Cω9、C10-甲基（TBSA）和总和特征 3 作为主要的细胞脂肪酸和 MK-8（H4ω-环）作为主要的呼吸醌。极性脂谱包括磷脂酰乙醇胺、双磷脂酰甘油、磷脂酰肌醇、磷脂酰肌醇甘露糖苷和未知的磷脂。细胞壁含有 -二氨基庚二酸，全细胞水解产物含有阿拉伯糖、半乳糖、葡萄糖、果糖、核糖和甘露糖。菌株 CFH S0054 的基因组为 7.9 Mbp，G+C 含量为 68.4 mol%，菌株 CFH S0054 和 IFM0354 之间的平均核苷酸同一性（ANI）值较低（ANI<87.7%，ANIb=84.1%）。基于系统发育、生理和化学分类学数据，菌株 CFHS0054 被认为代表了一个新的 属种，建议使用 sp. nov.来命名，其模式菌株为 CFH S0054（DSM 46763=JCM 30082）。
