Huang Jian-Rong, Ming Hong, Duan Yan-Yan, Li Shuai, Zhang Ling-Yu, Ji Wei-Li, Zhao Zhuo-Li, Meng Xiao-Lin, Li Wen-Jun, Nie Guo-Xing
College of Fisheries, Henan Normal University, Xinxiang, 453007, PR China.
Synthetic Biology Remaking Engineering and Application Laboratory (SynBreal), College of Life Sciences and Technology, Xinxiang Medical University, Xinxiang 453003, PR China.
Int J Syst Evol Microbiol. 2017 Sep;67(9):3467-3473. doi: 10.1099/ijsem.0.002143. Epub 2017 Sep 6.
A novel Gram-stain-positive, aerobic, non-motile and acid-fast actinomycete strain, designated CFH S0067T, was isolated from a soil sample collected from Heshun old town in Tengchong, Yunnan province, in south-west PR China. The taxonomic position of strain CFH S0067T was studied in detail using a polyphasic approach. Phylogenetic analysis, based on 16S rRNA gene sequences, revealed that strain CFH S0067T belongs to the genus Nocardia and is closely related to Nocardia concava JCM 12351T (99.3 % similarity), forming a separated branch with this type strain. However, the strain shared 96.0 % gyrB gene sequence similarity with N. concava JCM 12351T. Furthermore, DNA-DNA hybridization showed 56.5±0.6 % DNA relatedness between the novel strain and N. concava JCM 12351T. The whole-cell hydrolysates contained meso-diaminopimelic acid (type IV) and arabinose, galactose, fructose and mannose. The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside and one unidentified lipid. Strain CFH S0067T contained MK-8 (H4ω-cycl) as the predominant menaquinone. C16 : 0, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), C18 : 1ω9c and C18 : 0 10-methyl (TBSA) were the major cellular fatty acids. Mycolic acids were also detected. The G+C content of the genomic DNA was determined to be 66.9 mol%. A combination of the low DNA-DNA hybridization values and phenotypic properties demonstrated that strain CFH S0067Tis clearly distinguishable from its most closely related strain, N. concava JCM 12351T. On the basis of this polyphasic study, it is concluded that strain CFH S0067T should be considered to represent a novel species of the genus Nocardia, for which the name Nocardia heshunensis sp. nov. is proposed. The type strain is CFH S0067T (=DSM 46764T=JCM 30085T).
从中国西南部云南省腾冲和顺古镇采集的土壤样本中分离出一株新型革兰氏阳性、需氧、不运动且抗酸的放线菌菌株，命名为CFH S0067T。采用多相分类法对菌株CFH S0067T的分类地位进行了详细研究。基于16S rRNA基因序列的系统发育分析表明，菌株CFH S0067T属于诺卡氏菌属，与凹面诺卡氏菌JCM 12351T密切相关（相似性为99.3%），与该模式菌株形成一个独立分支。然而，该菌株与凹面诺卡氏菌JCM 12351T的gyrB基因序列相似性为96.0%。此外，DNA-DNA杂交显示新菌株与凹面诺卡氏菌JCM 12351T之间的DNA相关性为56.5±0.6%。全细胞水解产物含有内消旋二氨基庚二酸（IV型）以及阿拉伯糖、半乳糖、果糖和甘露糖。主要极性脂为双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰肌醇、磷脂酰肌醇甘露糖苷和一种未鉴定的脂质。菌株CFH S0067T含有MK-8（H4ω-环）作为主要甲基萘醌。C16 : 0、总和特征3（C16 : 1ω7c和/或C16 : 1ω6c）、C18 : 1ω9c和C18 : 0 10-甲基（TBSA）是主要的细胞脂肪酸。还检测到了分枝菌酸。基因组DNA的G+C含量测定为66.9 mol%。低DNA-DNA杂交值和表型特征的组合表明，菌株CFH S0067T与其最密切相关菌株凹面诺卡氏菌JCM 12351T明显不同。基于这项多相研究，得出结论，菌株CFH S0067T应被视为代表诺卡氏菌属的一个新物种，为此提出新名称和顺诺卡氏菌（Nocardia heshunensis sp. nov.）。模式菌株为CFH S0067T（=DSM 46764T=JCM 30085T）。
