Shamir Shelly K, Hagen Christopher R M, Foley Peter M, Gilroy Cornelia V, Yu Jenny, Amsellem Pierre M
Am J Vet Res. 2019 Aug;80(8):787-791. doi: 10.2460/ajvr.80.8.787.
To evaluate safety of stylet-in and stylet-out techniques for collection of CSF from the cisterna magna and to assess whether there were differences between techniques with regard to contamination of samples, sample quality, and efficiency of collection.
10 adult purpose-bred research Beagles.
A prospective crossover study was conducted. Preanesthetic physical and neurologic examinations and hematologic analyses were performed. Dogs were anesthetized, and collection of CSF samples from the cisterna magna by use of a stylet-in or stylet-out technique was performed. Two weeks later, samples were collected with the other sample collection technique. Samples of CSF were processed within 1 hour after collection.
Cellular debris was detected in higher numbers in stylet-in samples, although this did not affect sample quality. The stylet-out technique was performed more rapidly. No adverse effects were detected for either technique.
Both techniques could be safely performed in healthy anesthetized dogs. The stylet-out technique was performed more rapidly and yielded a sample with less cellular debris. Both techniques can be used in clinical practice to yield CSF samples with good diagnostic quality.
评估带芯针和无芯针技术从枕大池采集脑脊液的安全性，并评估两种技术在样本污染、样本质量和采集效率方面是否存在差异。
10只成年专用繁殖研究比格犬。
进行前瞻性交叉研究。进行麻醉前体格检查、神经学检查和血液学分析。对犬只进行麻醉，并使用带芯针或无芯针技术从枕大池采集脑脊液样本。两周后，用另一种样本采集技术采集样本。脑脊液样本在采集后1小时内进行处理。
带芯针样本中检测到的细胞碎片数量更多，尽管这并未影响样本质量。无芯针技术操作更快。两种技术均未检测到不良反应。
两种技术均可在健康麻醉犬中安全进行。无芯针技术操作更快，产生的样本细胞碎片更少。两种技术均可用于临床实践，以获得具有良好诊断质量的脑脊液样本。
