Wang Limei, Yang Wei, Ran Yanhong, Song Hui, Yan Xinxin, Guo Jianmin
Guangzhou Bay Area Institute of Biomedicine, Guangdong Lewwin Pharmaceutical Research Institute Co., Ltd., Guangdong Provincial Key Laboratory of Drug Non-Clinical Evaluation and Research, TCM Non-Clinic Evaluation Branch of National Engineering Research Center for Modernization of Traditional Chinese Medicine, Guangdong Engineering Research Center for Innovative Drug Evaluation and Research, Guangzhou 510990, China.
Division of Life Science and State Key Lab of Molecular Neuroscience, Hong Kong University of Science and Technology, Hong Kong 999077, China.
Vet Sci. 2025 Jan 15;12(1):58. doi: 10.3390/vetsci12010058.
The aim of this study was to explore an improved method for extracting rat cerebrospinal fluid (CSF), observing the impact on animal health under conditions of large-scale CSF collection and evaluating the feasibility of repeated collections. A total of 20 rats were anesthetized and fixed in a stereotactic frame. A 26G scalp needle, combined with a 1 mL syringe, was used to puncture the atlanto-occipital membrane and collect approximately 170 μL of CSF. CSF was collected twice within 14 days. During the study, animals were monitored daily for food intake, body weight, and hematological parameters, and at the end of the study, histopathological examination was performed. The health of the animals remained good, and repeated CSF collections were feasible. The success rate of the procedure was 100%, with blood contamination in the CSF decreasing from 70% in the first collection to 35% in the second. This technique is convenient, accurate, and suitable for widespread applications.
本研究的目的是探索一种改进的大鼠脑脊液（CSF）提取方法，观察大规模采集脑脊液条件下对动物健康的影响，并评估重复采集的可行性。总共20只大鼠被麻醉并固定在立体定位框架中。使用26G头皮针结合1 mL注射器穿刺枕寰膜并收集约170 μL脑脊液。在14天内收集脑脊液两次。在研究期间，每天监测动物的食物摄入量、体重和血液学参数，并在研究结束时进行组织病理学检查。动物健康状况良好，重复采集脑脊液是可行的。该操作的成功率为100%，脑脊液中的血液污染率从第一次采集时的70%降至第二次采集时的35%。该技术简便、准确，适合广泛应用。
