Slot J W, Strous G J, Geuze J J
J Cell Biol. 1979 Mar;80(3):708-14. doi: 10.1083/jcb.80.3.708.
Frog exocrine pancreatic tissue was studied in vitro under conditions which maintain the differences between tissues from fasted and fed animals. Sodium dodecyl sulfate (SDS) gel electrophoresis after labeling with [14C]amino acids showed that feeding stimulated the synthesis of secretory proteins to the same relative degree as the overall protein synthesis. The intracellular transport of secretory proteins was studied by electronmicroscopy autoradiography after pulse-labeling with [3H]leucine. It was found that the transport route is similar under both feeding conditions. After their synthesis in the rough endoplasmic reticulum (RER), the proteins move through the peripheral elements and cisternae of the Golgi system into the condensing vacuoles. The velocity of the transport increases considerably after feeding. When frogs are fasted, the release of labeled proteins from the RER takes greater than 90 min, whereas after feeding, this happens within 30 min. Comparable differences were observed for transport through the Golgi system. The apparent differences between the frog and mammalian pancreas in the regulation of synthesis, intracellular transport, and secretion of proteins are discussed.
在维持禁食和喂食动物组织差异的条件下，对青蛙的胰腺外分泌组织进行了体外研究。用[¹⁴C]氨基酸标记后进行十二烷基硫酸钠（SDS）凝胶电泳，结果表明，喂食对分泌蛋白合成的刺激程度与整体蛋白质合成的刺激程度相同。在用[³H]亮氨酸脉冲标记后，通过电子显微镜放射自显影研究了分泌蛋白的细胞内运输。结果发现，在两种喂食条件下，运输途径相似。这些蛋白质在粗面内质网（RER）中合成后，通过高尔基体系统的周边成分和潴泡进入浓缩液泡。喂食后运输速度显著增加。当青蛙禁食时，标记蛋白从RER释放需要超过90分钟，而喂食后，这一过程在30分钟内发生。在通过高尔基体系统的运输过程中也观察到了类似的差异。本文讨论了青蛙和哺乳动物胰腺在蛋白质合成、细胞内运输和分泌调节方面的明显差异。
