Slot J W, Geuze J J
Cell Tissue Res. 1976 Mar 16;167(2):147-65. doi: 10.1007/BF00224324.
Frog pancreatic tissue was pulse-labelled in vitro with 3H-leucine and protein transport was studied in exocrine cells by electron microscope autoradiography. The proteins appeared to be synthesized in the RER and transported to the secretory granules along a similar route and with the same velocity as previously described under in vitro conditions. Evidence was obtained for the involvement of the vesicular and tubular elements at the periphery of the Golgi system in transferring protein from the RER to the Golgi cisternae. Kinetics of the release of newly synthesized proteins from the RER and their appearance in the condensing vacuoles are discussed and related to results reported from other tissues. The transport velocity in this poikilothermic system was studied in relation to the incubation temperature and compared with results reported from its mammalian counterpart. At temperatures between 20 and 30 degrees C intracellular protein transport occurs faster in the frog than in the Guinea pig pancreas. At higher temperature the transport process was severely disturbed in the frog.
用³H-亮氨酸对青蛙胰腺组织进行体外脉冲标记，并通过电子显微镜放射自显影术研究外分泌细胞中的蛋白质转运。蛋白质似乎在粗面内质网（RER）中合成，并沿着与先前在体外条件下描述的相似途径、以相同速度转运至分泌颗粒。有证据表明，高尔基体系统周边的囊泡和管状成分参与了将蛋白质从粗面内质网转运至高尔基池的过程。讨论了新合成蛋白质从粗面内质网释放的动力学及其在浓缩泡中的出现情况，并与其他组织报道的结果相关联。研究了这个变温动物系统中的转运速度与孵育温度的关系，并与来自其哺乳动物对应物的结果进行了比较。在20至30摄氏度之间的温度下，青蛙细胞内蛋白质转运比豚鼠胰腺更快。在较高温度下，青蛙的转运过程受到严重干扰。
