El Gabbas Zineb, Bezza Kenza, Laadraoui Jawad, Ait Laaradia Mehdi, Kebbou Aaziz, Oufquir Sara, Boukhira Abderrahman, Aboufatima Rachida, Chait Abderrahman
Laboratory of Pharmacology, Neurobiology and Behavior, Semlalia Faculty of Sciences, Cadi Ayyad University, Marrakech, Morocco.
Biochemistry and Toxicology Department, Avicenna Military Hospital, Medical School Faculty, Cadi Ayyad University, Marrakech, Morocco.
Evid Based Complement Alternat Med. 2019 Jun 24;2019:1702378. doi: 10.1155/2019/1702378. eCollection 2019.
The leaves of . have a traditional reputation for the management of pain in Morocco. This study was conducted to investigate the curative effects of (SO) and its major constituents Rosmarinic (ROS) and Caffeic acids (CAF) on peripheral neuropathic pain in mice. Chronic constriction injury (CCI) was induced in mice, and neuropathic pain behaviors tests were evaluated by mechanical, chemical, thermal sensation tests and functional recovery of the sciatic nerve at different time intervals, ., (day 0, 1, 7, 14, and 21). Ethanolic extract of SO (100 and 200 mg/kg, ), ROS (10 and 20 mg/kg, ), CAF (30 and 40 mg/kg, ), and CLOM (5 mg/kg, , a positive control) was given for 21 days after surgery. Hematological and biochemical parameters were also measured as well as histopathological analysis. CCI produced significant development in mechanical and thermal hyperalgesia, cold allodynia, and rise in the sciatic functional index in mice. Chronic treatments with SO extract, ROS, CAF, and CLOM for 3 weeks significantly increased mechanical sensibility, cold, and thermal withdrawal latency and enhanced functional recovery of the injured nerve. The same treatments remarkably ameliorated hematological parameters and did not alter biochemical levels. The histopathological findings had revealed the protective effect of SO, ROS, and CAF against the CCI-induced damage. Our data support the use of SO in folk medicine to alleviate pain. Their main phenolic constituents could be promising antineuropathic compounds, which may be attributed to their biological activities including anti-inflammatory, antioxidant, and neuroprotective effects. SO leaves may be a good candidate to treat neuropathic pain.
在摩洛哥，[植物名称]的叶子在传统上就有治疗疼痛的声誉。本研究旨在调查[植物名称]提取物（SO）及其主要成分迷迭香酸（ROS）和咖啡酸（CAF）对小鼠周围神经性疼痛的治疗效果。对小鼠进行慢性缩窄损伤（CCI），并在不同时间间隔（即第0、1、7、14和21天）通过机械、化学、热感觉测试以及坐骨神经功能恢复情况来评估神经性疼痛行为测试。术后21天给予SO乙醇提取物（100和200毫克/千克，腹腔注射）、ROS（10和20毫克/千克，腹腔注射）、CAF（30和40毫克/千克，腹腔注射）以及氯氮平（5毫克/千克，腹腔注射，阳性对照）。同时还测量了血液学和生化参数，并进行了组织病理学分析。CCI导致小鼠出现明显的机械性和热性痛觉过敏、冷觉异常性疼痛以及坐骨神经功能指数升高。用SO提取物、ROS、CAF和氯氮平进行为期3周的慢性治疗显著提高了机械感觉、冷觉和热觉撤离潜伏期，并促进了受损神经的功能恢复。相同的治疗显著改善了血液学参数，且未改变生化水平。组织病理学结果显示SO、ROS和CAF对CCI诱导的损伤具有保护作用。我们的数据支持在民间医学中使用SO来缓解疼痛。它们的主要酚类成分可能是有前景的抗神经性化合物，这可能归因于它们的生物活性，包括抗炎、抗氧化和神经保护作用。SO叶可能是治疗神经性疼痛的良好候选药物。
