Bogner U, Sigle B, Schleusener H
Endocrine Department of the Medical Clinic, Free University of Berlin, Germany.
Immunobiology. 1988 Mar;176(4-5):423-31. doi: 10.1016/s0171-2985(88)80023-8.
Interferons have been shown to have antagonistic effects on cell-mediated cytotoxicity. In the present study, we investigated various types of immunologically mediated cytotoxic reactions with a 51chromium release assay, using human thyroid cells as targets, which were cultured in the presence and absence of interferon-gamma (IFN-gamma). Antibody-dependent cell-mediated cytotoxicity (ADCC), natural killer (NK) cell-mediated cytotoxicity, and autologous lymphocyte-mediated cytotoxicity (ALC) were measured utilizing thyroid tissue, sera and lymphocytes from patients with autoimmune thyroid disease as well as from normal subjects. ADCC, determined with sera from patients with Hashimoto's thyroiditis, was reduced by 70% when the thyroid target cells were cultured in the presence of 1000 U human IFN-gamma/ml culture medium. Similarly, natural killer cell-mediated cytotoxicity, as well as autologous lymphocyte-mediated cytotoxicity, was significantly reduced after pretreatment of thyroid target cells with 500-1000 U IFN-gamma/ml culture medium. Although it is known that IFN-gamma renders target cells resistant to NK cell-mediated lysis, this is the first report on this effect using human epithelial cells, which may have major implications for the suggested role of IFN-gamma in the induction and perpetuation of autoimmune thyroid disease.
干扰素已被证明对细胞介导的细胞毒性具有拮抗作用。在本研究中，我们使用人甲状腺细胞作为靶细胞，通过51铬释放试验研究了各种类型的免疫介导的细胞毒性反应，这些靶细胞在有和没有γ干扰素（IFN-γ）的情况下进行培养。利用自身免疫性甲状腺疾病患者以及正常受试者的甲状腺组织、血清和淋巴细胞，检测了抗体依赖性细胞介导的细胞毒性（ADCC）、自然杀伤（NK）细胞介导的细胞毒性和自体淋巴细胞介导的细胞毒性。当甲状腺靶细胞在含有1000 U人IFN-γ/毫升培养基的情况下培养时，用桥本甲状腺炎患者血清测定的ADCC降低了70%。同样，在用500 - 1000 U IFN-γ/毫升培养基预处理甲状腺靶细胞后，自然杀伤细胞介导的细胞毒性以及自体淋巴细胞介导的细胞毒性也显著降低。尽管已知IFN-γ使靶细胞对NK细胞介导的裂解产生抗性，但这是首次使用人上皮细胞报道这种效应，这可能对IFN-γ在自身免疫性甲状腺疾病的诱导和持续存在中所起的作用具有重要意义。
