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人类Rh血型系统中G抗原的生化鉴定与特性分析

Biochemical identification and characterization of the G antigen in the human blood group Rh.

作者信息

Lirochon J, Doinel C, Rouger P, Goossens D, Salmon C

机构信息

Fondation Nationale de Transfusion, Sanguine, Paris, France.

出版信息

Immunology. 1988 Jun;64(2):337-40.

PMID:3134298
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1384965/
Abstract

The human blood group Rh comprises erythrocyte membrane antigens which include the G antigen. In order to carry out this study, the G antigen was specifically immunofixed with a human monoclonal anti-Rh(G) antibody. After extraction and purification of the antigenic complex, a migration in SDS-PAGE showed the G antigen to be associated with a polypeptide of 28,000 MW.

摘要

人类Rh血型系统包含红细胞膜抗原，其中包括G抗原。为开展本研究，使用人源单克隆抗Rh(G)抗体对G抗原进行特异性免疫固定。抗原复合物经提取和纯化后，在SDS-PAGE中的迁移显示G抗原与一条分子量为28,000的多肽相关。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2fa9/1384965/29561708d222/immunology00154-0141-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2fa9/1384965/70e158e19ab4/immunology00154-0141-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2fa9/1384965/96e089077889/immunology00154-0141-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2fa9/1384965/29561708d222/immunology00154-0141-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2fa9/1384965/70e158e19ab4/immunology00154-0141-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2fa9/1384965/96e089077889/immunology00154-0141-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2fa9/1384965/29561708d222/immunology00154-0141-c.jpg

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本文引用的文献

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The preparation and chemical characteristics of hemoglobin-free ghosts of human erythrocytes.人红细胞无血红蛋白空泡的制备及其化学特性
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Human monoclonal antibodies against blood group antigens. Preparation of a series of stable EBV immortalized B clones producing high levels of antibody of different isotypes and specificities.抗血型抗原的人单克隆抗体。一系列稳定的EB病毒永生化B克隆的制备，这些克隆可产生高水平的不同同种型和特异性抗体。
J Immunol Methods. 1987 Aug 3;101(2):193-200. doi: 10.1016/0022-1759(87)90150-5.
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Human monoclonal antibody against Rh(D) antigen: partial characterization of the Rh(D) polypeptide from human erythrocytes.
Blood. 1987 May;69(5):1491-7.
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A sensitive method for estimating the carbohydrate content of glycoproteins.一种用于估算糖蛋白中碳水化合物含量的灵敏方法。
Anal Biochem. 1976 Feb;70(2):336-45. doi: 10.1016/0003-2697(76)90454-1.