实验性感染水牛（Bubalus bubalis）中抗李斯特菌溶血素O和单核细胞增生李斯特菌的检测

Detection of anti-listeriolysin O and Listeria monocytogenes in experimentally infected buffaloes (Bubalus bubalis).

作者信息

Chaudhari S P, Malik S V, Rekha G B, Barbuddhe S B

机构信息

Division of Veterinary Public Health, Indian Veterinary Research Institute, Izatnagar, UP, India.

出版信息

Trop Anim Health Prod. 2001 Jul;33(4):285-93. doi: 10.1023/a:1010579701464.

DOI:10.1023/a:1010579701464

PMID:11474862

Abstract

The kinetics of antibody production against listeriolysin O (ALLO) and the recovery pattern of Listeria monocytogenes from bacteriological samples were studied following oral infection of buffalo calves with 3 x 10(9) cells each of pathogenic L. monocytogenes. Antibodies to LLO appeared by 7-10 days post infection (PI), with a shallow peak between days 16 and 36 PI, when tested by indirect plate-ELISA. The titres of ALLO in all the animals then declined slowly but remained detectable up to day 70 PI. In dot-ELISA, ALLO could be detected by days 5 to 7 PI, and with higher titres than with the plate-ELISA. The pathogen was recovered at low rates as ALLO first appeared but was absent in the faecal, nasal and blood cultures as production of ALLO peaked.

摘要

在用3×10⁹个致病性单核细胞增生李斯特菌细胞分别口服感染水牛犊后，研究了针对李斯特菌溶血素O（ALLO）的抗体产生动力学以及从细菌学样本中回收单核细胞增生李斯特菌的模式。通过间接平板ELISA检测，感染后7 - 10天出现针对LLO的抗体，在感染后16至36天之间出现一个浅峰。之后所有动物体内ALLO的滴度缓慢下降，但直到感染后70天仍可检测到。在斑点ELISA中，感染后5至7天可检测到ALLO，且滴度高于平板ELISA。随着ALLO首次出现，病原体以低比率被回收，但在粪便、鼻腔和血液培养物中，当ALLO产生达到峰值时则未检测到。