Department of Biosciences, University of Oslo, Oslo, Norway.
Environ Toxicol Chem. 2019 Oct;38(10):2224-2232. doi: 10.1002/etc.4541.
Copper (Cu) is an essential trace metal, but may also be toxic to aquatic organisms. Although many studies have investigated the cytotoxicity of Cu, little is known about the in vivo genotoxic potential of Cu in marine invertebrates. We investigated the genotoxicity of Cu in 2 pelagic calanoid copepods, Acartia tonsa and Temora longicornis, and the intertidal harpacticoid copepod Tigriopus brevicornis by exposing them for 6 and 72 h to waterborne Cu (0, 6, and 60 µg Cu/L). A subsequent 24-h period in filtered seawater was used to investigate delayed effects or recovery. Genotoxicity was evaluated as DNA strand breaks in individual copepods using the comet assay. Copper did not increase DNA strand breaks in any of the species at any concentration or time point. The treatment did, however, cause 100% mortality in A. tonsa following exposure to 60 µg Cu/L. Acartia tonsa and T. longicornis were more susceptible to Cu-induced mortality than the benthic harpacticoid T. brevicornis, which appeared to be unaffected by the treatments. The results show major differences in Cu susceptibility among the 3 copepods and also that acute toxicity of Cu to A. tonsa is not directly associated with genotoxicity. We also show that the comet assay can be used to quantify genotoxicity in individual copepods. Environ Toxicol Chem 2019;38:2224-2232. © 2019 SETAC.
铜（Cu）是一种必需的微量元素，但也可能对水生生物有毒。尽管许多研究已经调查了 Cu 的细胞毒性，但对于海洋无脊椎动物中 Cu 的体内遗传毒性潜力知之甚少。我们通过将 2 种浮游桡足类桡足类，A. tonsa 和 Temora longicornis，以及潮间带桡足类桡足类 Tigriopus brevicornis 暴露于水载 Cu（0、6 和 60μg Cu/L）中 6 和 72 小时，研究了 Cu 的遗传毒性。随后在过滤海水中进行了 24 小时的后续处理，以研究延迟效应或恢复情况。通过彗星试验评估了个体桡足类动物的 DNA 链断裂作为遗传毒性。在任何浓度或时间点，Cu 都不会增加任何物种的 DNA 链断裂。然而，在用 60μg Cu/L 处理后，A. tonsa 的处理导致 100%的死亡率。A. tonsa 和 T. longicornis 比底栖桡足类 T. brevicornis 更容易受到 Cu 诱导的死亡率，而 T. brevicornis 似乎不受处理的影响。结果表明，这 3 种桡足类动物对 Cu 的敏感性存在很大差异，并且 A. tonsa 对 Cu 的急性毒性与遗传毒性没有直接关系。我们还表明，彗星试验可用于量化个体桡足类动物的遗传毒性。Environ Toxicol Chem 2019;38:2224-2232。 © 2019 SETAC。
