Department of Chemical and Biological Engineering, Korea University, 145, Anam-ro, Seoungbuk-gu, Seoul 02841, Republic of Korea.
Department of Chemical and Biological Engineering, Korea University, 145, Anam-ro, Seoungbuk-gu, Seoul 02841, Republic of Korea.
Bioresour Technol. 2019 Nov;291:121820. doi: 10.1016/j.biortech.2019.121820. Epub 2019 Jul 16.
Mixotrophy (M) assumes sum of autotrophic (A) and heterotrophic (H) growths. In this study, a novel split-mixotrophic cultivation strategy (SMCS) developed as better mixotrophy via offering mutual-benefits through gas-exchange at both headspaces while splitting both trophic modes. To quantify synergistic-growth effects in combined-autotrophy and combined-heterotrophy (CA&CH) of SMCS, gross O-evolution, DIC and DO concentrations were compared with A, H and M. Average 12-14% and 26-32% increase in DIC and DO concentrations were determined respectively in CA and CH than A, H and M. Biomass yield in CA + CH was increased approx.1.5-folds higher than yields of A + H and M regimes. These results show SMCS as better cultivation strategy than the M by increased biomass and lipid yields. Challenges associated with organic carbon can be solved by SMCS viz. chlorophyll loss, organic carbon uptake inhibition. SMCS could be a breakthrough to integrate bacterial process with algae for better bioprocess economy and energy recovery.
混养（M）假定自养（A）和异养（H）生长的总和。在这项研究中，开发了一种新型的分裂混养培养策略（SMCS），通过在两个气腔进行气体交换来提供互利，从而实现更好的混养。为了量化 SMCS 中组合自养和组合异养（CA&CH）的协同生长效应，比较了总 O 演化、DIC 和 DO 浓度与 A、H 和 M 的差异。与 A、H 和 M 相比，CA 和 CH 中的 DIC 和 DO 浓度分别平均增加了 12-14%和 26-32%。CA+CH 中的生物量产量比 A+H 和 M 培养的生物量产量增加了约 1.5 倍。这些结果表明，与 M 相比，SMCS 通过增加生物量和脂质产量，是一种更好的培养策略。SMCS 可以解决与有机碳相关的挑战，例如叶绿素损失、有机碳吸收抑制。SMCS 可能是将细菌过程与藻类结合起来以实现更好的生物工艺经济性和能源回收的突破。
