Physikalisches Institut and Center for Soft Nanoscience, Westfälische Wilhelms-Universität Münster, Busse-Peus-Strasse 10, 48149 Münster, Germany.
Biofabrication. 2019 Aug 22;11(4):045015. doi: 10.1088/1758-5090/ab3582.
In this study, we report the step-gradient nanocomposite (NC) hydrogel generated easily by spatial connection of different nanocomposite hydrogel pastes varying in the concentrations of nanomaterials with the aid of a 3D printing technique. The prepared 3D printed gradient NC hydrogel has self-adhesive properties and is used to direct the migration of fibroblast cells towards the higher concentration of biopolymer-coated silica-based nanomaterials (NMs) within the 3D network of the hydrogel. Furthermore, we demonstrate the potential application of our gradient NC hydrogel in migration and subsequent enhanced osteogenic differentiation of human bone marrow derived mesenchymal stem cells (hBM MSC). The osteogenic differentiation of hBM MSC is achieved in the absence of osteogenic differentiation medium due to the silica-based NMs. The increase in the NM content in the gradient construct promotes hBM MSC migration and results in higher Ca deposition.
在这项研究中，我们报告了一种通过空间连接不同浓度纳米复合材料水凝胶的方法来制备具有台阶梯度的纳米复合水凝胶。该方法借助 3D 打印技术，将纳米复合材料水凝胶的浓度逐渐变化。所制备的 3D 打印梯度纳米复合水凝胶具有自粘性，并可用于引导成纤维细胞向水凝胶 3D 网络中的更高浓度的生物聚合物涂覆的基于二氧化硅的纳米材料（NMs）迁移。此外，我们还展示了我们的梯度纳米复合水凝胶在人骨髓间充质干细胞（hBM MSC）迁移和随后增强成骨分化中的潜在应用。由于基于二氧化硅的纳米材料的存在，hBM MSC 的成骨分化可以在没有成骨分化培养基的情况下实现。梯度构建中 NM 含量的增加促进了 hBM MSC 的迁移，并导致更高的 Ca 沉积。
