Nirwan Viraj P, Al-Kattan Ahmed, Fahmi Amir, Kabashin Andrei V
Faculty of Technology and Bionics, Rhine-Waal University of Applied Science, Marie-Curie-Straβe 1, 47533 Kleve, Germany.
Aix Marseille University, CNRS, LP3 (UMR 7341), 13288 Marseille, France.
Nanomaterials (Basel). 2019 Jul 24;9(8):1058. doi: 10.3390/nano9081058.
We report a methodology for the fabrication of neutralized chitosan-based nanofiber matrices decorated with bare Au nanoparticles, which demonstrate stable characteristics even after prolonged contact with a biological environment. The methodology consists of electrospinning of a mixture of bare (ligand-free) laser-synthesized Au nanoparticles (AuNPs) and solutions of chitosan/polyethylene oxide (ratio 1/3) containing chitosan of a relatively high molecular weight (200 kDa) and concentration of 3% (/). Our studies reveal a continuous morphology of hybrid nanofibers with the mean fiber diameter of 189 nm ± 86 nm, which demonstrate a high thermal stability. Finally, we describe a protocol for the neutralization of nanofibers, which enabled us to achieve their structural stability in phosphate-buffered saline (PBS) for more than six months, as confirmed by microscopy and FTIR measurements. The formed hybrid nanofibers exhibit unique physicochemical properties essential for the development of future tissue engineering platforms.
我们报告了一种制备用裸金纳米颗粒修饰的中和壳聚糖基纳米纤维基质的方法，即使在与生物环境长时间接触后，该基质仍表现出稳定的特性。该方法包括静电纺丝裸（无配体）激光合成的金纳米颗粒（AuNPs）与壳聚糖/聚环氧乙烷（比例为1/3）的溶液的混合物，其中壳聚糖具有相对较高的分子量（200 kDa）且浓度为3%（/）。我们的研究揭示了平均纤维直径为189 nm±86 nm的混合纳米纤维的连续形态，其表现出高热稳定性。最后，我们描述了一种纳米纤维中和方案，通过显微镜和傅里叶变换红外光谱（FTIR）测量证实，该方案使我们能够在磷酸盐缓冲盐水（PBS）中实现纳米纤维超过六个月的结构稳定性。所形成的混合纳米纤维展现出对未来组织工程平台发展至关重要的独特物理化学性质。
