Kim Han-Na, Joo Eun-Jeong, Cheong Hae Suk, Kim Yejin, Kim Hyung-Lae, Shin Hocheol, Chang Yoosoo, Ryu Seungho
Medical Research Institute, Kangbuk Samsung Hospital, Sungkyunkwan University, School of Medicine, Seoul 03181, Korea.
Center for Cohort Studies, Total Healthcare Center, Kangbuk Samsung Hospital, Sungkyunkwan University, School of Medicine, Seoul 04514, Korea.
J Clin Med. 2019 Jul 24;8(8):1089. doi: 10.3390/jcm8081089.
Gut dysbiosis is regarded as a pathogenetic factor of nonalcoholic fatty liver disease (NAFLD), but its role in NAFLD persistence is unknown. We investigated the influence of the gut microbiota on persistent NAFLD. This cohort study included 766 subjects with 16S ribosomal RNA (rRNA) gene sequencing data from fecal samples at baseline who underwent repeated health check-up examinations. Fatty liver was determined using ultrasound at baseline and follow-up. Participants were categorized into four groups: none (control), developed, regressed, or persistent NAFLD. The persistent NAFLD group had lower richness compared with the control group. Significant differences were also found in both non-phylogenic and phylogenic beta diversity measures according to NAFLD persistence. Pairwise comparisons indicated that taxa abundance mainly differed between the control and persistent NAFLD groups. A relative high abundance of Fusobacteria and low abundance of genera and of the family Ruminococcaceae and genus of the family Lachnospiraceae were found in the persistent NAFLD group. Based on the functional predictions, pathways related to primary and secondary bile acid biosynthesis were highly detected in the persistent NAFLD group compared with the control group. These findings support that the composition of the gut microbiome associated with dysregulation of bile acid biosynthetic pathways may contribute to the persistence of NAFLD. This is the first cohort study to demonstrate the influence of microbiota on persistent NAFLD. Our findings may help identify potential targets for therapeutic intervention in NAFLD.
肠道菌群失调被认为是非酒精性脂肪性肝病（NAFLD）的一个致病因素，但其在NAFLD持续存在中的作用尚不清楚。我们研究了肠道微生物群对持续性NAFLD的影响。这项队列研究纳入了766名受试者，他们在基线时拥有粪便样本的16S核糖体RNA（rRNA）基因测序数据，并接受了多次健康检查。在基线和随访时使用超声确定脂肪肝情况。参与者被分为四组：无（对照组）、新发、消退或持续性NAFLD。与对照组相比，持续性NAFLD组的丰富度较低。根据NAFLD的持续性，在非系统发育和系统发育β多样性测量中也发现了显著差异。成对比较表明，分类群丰度主要在对照组和持续性NAFLD组之间有所不同。在持续性NAFLD组中发现梭杆菌相对丰度较高，瘤胃球菌科的属和毛螺菌科的属丰度较低。基于功能预测，与初级和次级胆汁酸生物合成相关的途径在持续性NAFLD组中比对照组中被高度检测到。这些发现支持与胆汁酸生物合成途径失调相关的肠道微生物组组成可能导致NAFLD的持续存在。这是第一项证明微生物群对持续性NAFLD影响的队列研究。我们的发现可能有助于确定NAFLD治疗干预的潜在靶点。
