State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, China; Center of Materials Science and Optoelectronics Engineering, University of Chinese Academy of Sciences, Beijing 100049, China.
State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, China.
J Trace Elem Med Biol. 2019 Sep;55:127-135. doi: 10.1016/j.jtemb.2019.06.015. Epub 2019 Jun 19.
Copper has already been widely used in the modification of biomaterials because it possesses multifunctional biological effects like osteogenic, angiogenic and antibacterial activities. However, it is still not clear how different cell lines and bacteria will respond to different concentrations of Cu, which is very critical to the application of copper-doped implants.
This study aimed to explore the dose-response relationships of Cu and its biological effects in vitro. Rat bone marrow mesenchymal stem cell (rBMSCs), mouse osteoblastic cell line (MC3T3-E1), and human umbilical vein endothelial cells (HUVECs) were used to evaluate cellular behaviors. Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) were used to evaluate bacterial behaviors.
Results showed that the HUVECs exhibited significantly higher tolerance to copper ions than MC3T3-E1 and rBMSCs. The IC values of copper for HUVECs, MC3T3-E1 and HUVECs were approximated to 327.9 μM, 134.6 μM, and 0.7 μM, respectively. Besides, the threshold concentration of copper for effective inhibition against bacteria growth is 37 μM. When the concentration exceeded the threshold value, antibacterial activity could increase dramatically.
These results altogether establish a technological foundation for the application of copper-doped biomaterials in bone growth and remodeling.
铜由于具有成骨、血管生成和抗菌等多种生物功能，已广泛应用于生物材料的改性。然而，不同细胞系和细菌对不同浓度铜的反应如何，对于铜掺杂植入物的应用至关重要，目前仍不清楚。
本研究旨在探索体外铜的剂量反应关系及其生物学效应。选用大鼠骨髓间充质干细胞（rBMSCs）、小鼠成骨细胞系（MC3T3-E1）和人脐静脉内皮细胞（HUVECs）评估细胞行为，选用金黄色葡萄球菌（S. aureus）和大肠杆菌（E. coli）评估细菌行为。
结果表明，HUVECs 对铜离子的耐受性明显高于 MC3T3-E1 和 rBMSCs。HUVECs、MC3T3-E1 和 HUVECs 的铜 IC 值分别接近 327.9µM、134.6µM 和 0.7µM。此外，铜对细菌生长有效抑制的阈值浓度为 37µM。当浓度超过阈值时，抗菌活性可显著增加。
这些结果为铜掺杂生物材料在骨生长和重塑中的应用奠定了技术基础。
