BUASMV "King Michael I of Romania" from Timisoara, Faculty of Veterinary Medicine, Romanian Society for Trace Elements in Medicine, Romania.
Working Group for Xenobiochemistry, Romanian Academy-Branch Timisoara, Romania.
J Trace Elem Med Biol. 2019 Sep;55:136-142. doi: 10.1016/j.jtemb.2019.06.013. Epub 2019 Jun 27.
Elderly individuals are exposed to trace element imbalances due to the reduced capacity of their organism to utilize minerals in a direct relationship with many circumstances.
The aim of this study was to assess the protective role of resveratrol upon the homeostatic changes of some trace elements in geriatric rats in the condition of oxidative stress induced by aluminum exposure. Forty Wistar rats, 18-20 months old, were divided randomly into four groups (n = 10): control (C) - receiving 1 ml of physiologically saline (P.S.) via intraperitoneal (i.p) administration, E1 - 1 ml of P.S. and 1000 ppb aluminum sulphate (AS) in drinking water ad libitum, E2 - 20 mg/kg resveratrol, i.p. and 1000 ppb AS in drinking water, E3 - 20 mg/kg resveratrol i.p. The groups C and E3 received distilled water as drinking water ad libitum. The i.p administrations were once a week for four weeks period. The levels of oxidative stress marker's were analyzed (glutathione, glutathione' peroxidase, superoxide dismutase, and catalase) of the proteins' (total protein, albumin, and hemoglobin) in serum and also the levels of the main trace elements (copper, zinc, iron, selenium, manganese and magnesium) in blood, liver, kidney and spleen.
Significant decrease (p < 0.05) of total protein (TP), albumin (ALB), catalase (CAT), increase significant (p < 0.05) of glutathione reductase (GSH-r), glutathione peroxidase (GPx) and superoxide dismutase (SOD) in E1 groups, compared with control, E2, and E3 groups was ascertained. There were also observed significant (p < 0.05) decreases in Cu, Zn, Fe and Mg, not significant (p > 0.05) increase of Se and Mn in blood, significant (p < 0.01) increase of Cu, Zn, Mg, Se, Mn in kidney and liver and Fe, in spleen of geriatric rats from E1 group compared to the control group. Insignificant differences (p > 0.05) were recorded in groups which received resveratrol (E2 and E3) compared to the control group, but significant differences (p < 0.05), especially in blood and liver samples, compared to E1.
The results suggest that resveratrol can prevent the homeostatic imbalance of trace elements in geriatric rats in the condition of oxidative stress induced by aluminum exposure.
由于老年人的机体利用矿物质的能力下降，他们可能会出现微量元素失衡，这与许多情况有关。
本研究旨在评估白藜芦醇对铝暴露诱导的氧化应激状态下老年大鼠体内某些微量元素的内稳态变化的保护作用。将 40 只 18-20 个月大的 Wistar 大鼠随机分为四组（n=10）：对照组（C）-通过腹腔内（i.p.）给予 1ml 生理盐水（P.S.），E1 组-给予 1ml P.S.和 1000ppb 硫酸铝（AS）自由饮水，E2 组-20mg/kg 白藜芦醇，i.p.和 1000ppb AS 自由饮水，E3 组-20mg/kg 白藜芦醇 i.p.，各组均给予蒸馏水自由饮水。每周腹腔注射一次，共四周。分析血清中氧化应激标志物（谷胱甘肽、谷胱甘肽过氧化物酶、超氧化物歧化酶和过氧化氢酶）和血液、肝脏、肾脏和脾脏中主要微量元素（铜、锌、铁、硒、锰和镁）的水平。
与对照组相比，E1 组总蛋白（TP）、白蛋白（ALB）、过氧化氢酶（CAT）显著降低（p<0.05），谷胱甘肽还原酶（GSH-r）、谷胱甘肽过氧化物酶（GPx）和超氧化物歧化酶（SOD）显著升高（p<0.05）。与对照组相比，E1 组血液中 Cu、Zn、Fe 和 Mg 显著降低（p<0.05），Se 和 Mn 无显著升高（p>0.05），肾脏和肝脏中 Cu、Zn、Mg、Se、Mn 显著升高（p<0.01），脾脏中 Fe 显著升高（p<0.01）。与对照组相比，给予白藜芦醇的 E2 和 E3 组无显著差异（p>0.05），但与 E1 组相比，尤其是在血液和肝脏样本中，差异显著（p<0.05）。
结果表明，白藜芦醇可预防铝暴露诱导的氧化应激状态下老年大鼠微量元素内稳态失衡。
