Gao Z, Mackenzie I C, Rittman B R, Korszun A K, Williams D M, Cruchley A T
Dow's Institute for Dental Research, University of Iowa, Iowa City 52242.
J Oral Pathol. 1988 Feb;17(2):84-90. doi: 10.1111/j.1600-0714.1988.tb01512.x.
Monoclonal antibodies (mAbs) were used to determine the presence and distribution of immune cells including lymphocytes, macrophages and Langerhans cells, in normal periodontal ligament, periapical granulomata, periapical cysts and dental developmental cysts. Isolated T-lymphocytes, but not B-lymphocytes, were detected in specimens of non-inflamed periodontal ligament. Increased numbers of T and B lymphocytes were found in all of the lesions examined. Monocytes/macrophages were associated with most periapical granulomata, dental developmental cysts and all periapical cysts. Langerhans cells, intraepithelial lymphocytes, and monocytes/macrophages were not detected in the rests of Malassez but were found in some epithelia within periapical granulomata and in most epithelial linings of odontogenic cysts. Increased numbers of immune cells were seen around proliferative epithelia and adjacent to the epithelial linings of cysts. Epithelium, particularly that of odontogenic cysts, showed positive reactions for HLA-Dr, lysozyme and for alpha-1 antitrypsin. The presence of immune cells in periapical granulomata and odontogenic cysts, suggests that cell-mediated and humoral immunoreactions occur in these lesions and may be associated with the epithelial proliferation within the periapical lesions.
采用单克隆抗体（mAbs）来确定免疫细胞，包括淋巴细胞、巨噬细胞和朗格汉斯细胞，在正常牙周膜、根尖肉芽肿、根尖囊肿和牙发育囊肿中的存在及分布情况。在未发炎的牙周膜标本中检测到了分离的T淋巴细胞，但未检测到B淋巴细胞。在所有检查的病变中均发现T淋巴细胞和B淋巴细胞数量增加。单核细胞/巨噬细胞与大多数根尖肉芽肿、牙发育囊肿以及所有根尖囊肿相关。在马拉瑟上皮剩余中未检测到朗格汉斯细胞、上皮内淋巴细胞和单核细胞/巨噬细胞，但在根尖肉芽肿内的一些上皮以及大多数牙源性囊肿的上皮衬里中发现了这些细胞。在增殖上皮周围以及囊肿上皮衬里附近可见免疫细胞数量增加。上皮，尤其是牙源性囊肿的上皮，对HLA-Dr、溶菌酶和α-1抗胰蛋白酶呈阳性反应。根尖肉芽肿和牙源性囊肿中存在免疫细胞，提示在这些病变中发生了细胞介导的和体液免疫反应，并且可能与根尖病变内的上皮增殖有关。
