Li T J, Browne R M, Matthews J B
Unit of Oral Pathology, School of Dentistry, University of Birmingham, England.
J Oral Pathol Med. 1994 Apr;23(4):184-9. doi: 10.1111/j.1600-0714.1994.tb01110.x.
The aim of this study was to investigate the reactivity of the epithelial linings of the three major types of odontogenic cyst with a monoclonal antibody to proliferating cell nuclear antigen (PCNA; clone PC10). PCNA expression was studied in odontogenic cysts (n = 31) and normal oral epithelium (n = 10) using a biotin-streptavidin method on routinely processed paraffin sections. PCNA+ cells were counted manually and related to the length of basement membrane (mm) and the epithelial area (mm2) as determined by TV image analysis. The epithelial linings of odontogenic keratocysts (OKC; n = 11) contained the highest number of PCNA+ cells, most of which were located in the suprabasal layers. The mean value of PCNA+ cells in OKC linings (94.4 +/- 22.7 cells/mm) was similar to that of oral epithelia (80.8 +/- 20.6 cells/mm), but both were significantly higher than that of dentigerous (n = 10, 5.1 +/- 3.0 cells/mm) and radicular (n = 10, 11.0 +/- 4.1 cells/mm) cyst linings (P < 0.005). The epithelial distribution of PCNA+ cells differed between groups with the basal/suprabasal PCNA+ cell ratio in OKC linings (0.05 +/- 0.02) being significantly lower than that of normal oral epithelium (0.5 +/- 0.14), dentigerous (1.6 +/- 1.23) and radicular (1.9 +/- 1.09) cyst linings respectively (P < 0.005). These results demonstrate differences in PCNA expression between the epithelial linings of the major odontogenic cyst types, indicating differences in proliferative and differentiation processes within these lesions.
本研究的目的是使用抗增殖细胞核抗原（PCNA；克隆PC10）的单克隆抗体，研究三种主要类型牙源性囊肿的上皮衬里的反应性。采用生物素-链霉亲和素法，在常规处理的石蜡切片上，对牙源性囊肿（n = 31）和正常口腔上皮（n = 10）中的PCNA表达进行研究。通过电视图像分析确定基底膜长度（mm）和上皮面积（mm2），并手动计数PCNA+细胞。牙源性角化囊肿（OKC；n = 11）的上皮衬里中PCNA+细胞数量最多，其中大多数位于基底上层。OKC衬里中PCNA+细胞的平均值（94.4±22.7个细胞/mm）与口腔上皮（80.8±20.6个细胞/mm）相似，但均显著高于含牙囊肿（n = 10，5.1±3.0个细胞/mm）和根尖囊肿（n = 10，11.0±4.1个细胞/mm）衬里中的值（P < 0.005）。PCNA+细胞的上皮分布在各组之间存在差异，OKC衬里中基底/基底上层PCNA+细胞的比例（0.05±0.02）显著低于正常口腔上皮（0.5±0.14）、含牙囊肿（1.6±1.23）和根尖囊肿（1.9±1.09）衬里中的比例（P < 0.005）。这些结果表明，主要牙源性囊肿类型的上皮衬里中PCNA表达存在差异，提示这些病变内增殖和分化过程存在差异。
