KU Leuven (University of Leuven), Department of Oral Health Sciences, BIOMAT - Biomaterials Research Group & UZ Leuven (University Hospitals Leuven), Dentistry, Leuven, Belgium.
University of Birmingham School of Dentistry, College of Medical and Dental Sciences, Institute of Clinical Sciences, Birmingham, United Kingdom.
Dent Mater. 2019 Sep;35(9):1342-1350. doi: 10.1016/j.dental.2019.07.004. Epub 2019 Jul 23.
The aim of this study was to evaluate the bioactivity potential of an hydraulic calcium-silicate cement, Pure Portland Cement Med-PZ (Medcem, Weinfelden, Switzerland: 'MPC'), applied in a tooth extracted because of failed regenerative endodontic procedures (REP) and by means of ex vivo (EV) specimens.
Ten EV cylindrical dentin cavities were prepared and filled with MPC and stored for 1 month in distilled water (DW), Hank's balanced salt solution (HBSS), Dulbecco's phosphate-buffered saline (DPBS), simulated body fluid (SBF), versus no media (NM) serving as control. Six additional EV specimens were filled with MPC and exposed for 2 weeks to leucocyte-and-platelet-rich fibrin (LPRF)-clot (C), LPRF-membrane (M) and LPRF-exudate (E). MPC in the EV specimens and in the coronal part of the REP tooth was analyzed by means of micro-Raman spectroscopy (MR), scanning electron microscopy (SEM) and energy dispersive spectroscopy (EDS).
SEM showed rough crystallite surfaces for the EV samples and a porous surface for the REP tooth. EDS of the EV samples revealed prominent peaks for Ca, Si and O. Storage in HBSS, DPBS, SBF, exposure to LPRF and the REP tooth showed considerable amounts of P as well. MR exhibited vibrations of phosphate (DPBS, SBF), carbonated hydroxyapatite (DPBS, SBF), calcium carbonate (DW, HBSS, NM, REP-tooth, LPRF-E), oxidized (ferric) proteins (LPRF-E/C/M) and the amide III band (all samples). Hence, only storage of MPC in DPBS and SBF for 1 month revealed bioactivity.
The environmental conditions, namely the laboratory and clinical settings, affect the bioactivity potential of MPC.
本研究旨在评估一种水硬性硅酸钙水泥——纯波特兰水泥 Med-PZ（瑞士威芬登的 Medcem，简称“MPC”）的生物活性潜力，该水泥应用于因再生内根管治疗（REP）失败而拔除的牙齿，并通过离体（EV）标本进行研究。
制备 10 个 EV 圆柱形牙本质腔，并用 MPC 填充，并在蒸馏水中（DW）、汉克平衡盐溶液（HBSS）、杜尔贝科磷酸盐缓冲盐水（DPBS）、模拟体液（SBF）中储存 1 个月，以无介质（NM）作为对照。另外 6 个 EV 标本用 MPC 填充，并暴露于富含白细胞和血小板的纤维蛋白（LPRF）-凝块（C）、LPRF-膜（M）和 LPRF-渗出物（E）中 2 周。通过微拉曼光谱（MR）、扫描电子显微镜（SEM）和能谱（EDS）分析 EV 标本和 REP 牙的 MPC。
SEM 显示 EV 样本的结晶表面粗糙，REP 牙的表面多孔。EDS 分析显示 EV 样本中 Ca、Si 和 O 含量较高。在 HBSS、DPBS、SBF 中储存、暴露于 LPRF 以及 REP 牙中，也发现了大量的 P。MR 显示 DPBS、SBF 中存在磷酸盐（DPBS、SBF）、碳酸羟基磷灰石（DPBS、SBF）、碳酸钙（DW、HBSS、NM、REP 牙、LPRF-E）、氧化（三价铁）蛋白（LPRF-E/C/M）和酰胺 III 带（所有样本）的振动。因此，只有将 MPC 在 DPBS 和 SBF 中储存 1 个月，才显示出生物活性。
环境条件，即实验室和临床环境，会影响 MPC 的生物活性潜力。
