Institute of Crop Science, National Agriculture and Food Research Organization (NARO), Tsukuba 305-8518, Japan.
Toyama Prefectural Agricultural, Forestry and Fisheries Research Center, Toyama 939-8153, Japan.
Science. 2019 Jul 26;365(6451):393-396. doi: 10.1126/science.aax0379.
The genetic variation of rice cultivars provides a resource for further varietal improvement through breeding. Some rice varieties are sensitive to benzobicyclon (BBC), a β-triketone herbicide that inhibits 4-hydroxyphenylpyruvate dioxygenase (HPPD). Here we identify a rice gene, (), that confers resistance to BBC and other β-triketone herbicides. We show that encodes an Fe(II)/2-oxoglutarate-dependent oxygenase that detoxifies β-triketone herbicides by catalyzing their hydroxylation. Genealogy analysis revealed that BBC-sensitive rice variants inherited a dysfunctional allele from an rice variety. Forced expression of in conferred resistance not only to BBC but also to four additional β-triketone herbicides. may prove useful for breeding herbicide-resistant crops.
水稻品种的遗传变异为通过育种进一步改良品种提供了资源。一些水稻品种对苯并双环酮（BBC）敏感，BBC 是一种β-三酮除草剂，能抑制 4-羟基苯基丙酮酸双加氧酶（HPPD）。在这里，我们鉴定了一个赋予水稻 BBC 及其他β-三酮除草剂抗性的基因， （）。我们表明， 编码一种依赖 Fe(II)/2-氧戊二酸的加氧酶，通过催化β-三酮除草剂的羟化反应来解毒。系统发育分析表明，BBC 敏感的水稻变种从一种 水稻品种继承了一个功能失调的 等位基因。在 中强制表达 不仅赋予了 BBC 抗性，还赋予了另外四种β-三酮除草剂抗性。 可能有助于培育抗除草剂作物。
