Clark Logan C, Gaglianese-Woody Morgan R, Estep Matt C
Department of Biology Appalachian State University 572 Rivers Street Boone North Carolina 28607-2027 USA.
Appl Plant Sci. 2019 May 17;7(7):e01250. doi: 10.1002/aps3.1250. eCollection 2019 Jul.
Microsatellite markers were developed in the federally endangered species (Asteraceae) to evaluate species boundaries with closely related congeners within the genus.
Using Illumina data, 17 primer pairs were developed in populations of . The primers amplified motifs from tri- to hexanucleotide repeats with one to 17 alleles per locus. Primers were also tested for cross-amplification in , , and .
The developed primers for serve as a novel genetic tool for future investigations in this genus, allowing for more explicit species delineation as well as population genetic analyses.
在联邦濒危物种（菊科）中开发了微卫星标记，以评估该属内与近缘同属物种的物种界限。
利用Illumina数据，在……种群中开发了17对引物。这些引物扩增了三核苷酸至六核苷酸重复序列的基序，每个位点有1至17个等位基因。还对引物在……、……和……中的交叉扩增进行了测试。
为……开发的引物可作为该属未来研究的一种新型遗传工具，有助于更明确地划分物种以及进行种群遗传分析。
