描述 12 个 Hypochaeris chillensis（菊科）微卫星位点的特征，并在相关物种中进行交叉扩增。
Characterization of 12 microsatellite loci for Hypochaeris chillensis (Asteraceae) and cross-amplification in related species.
机构信息
Departamento de Biologia Geral, Centro de Ciências Biológicas, Universidade Estadual de Londrina, 86051-990 Londrina, Paraná, Brazil.
出版信息
Am J Bot. 2011 Sep;98(9):e262-4. doi: 10.3732/ajb.1100177. Epub 2011 Aug 29.
PREMISE OF THE STUDY
Hypochaeris is considered a biological model to understand evolutionary processes in the vascular flora of South America, particularly from the temperate portion of the continent. We report the development and characterization of microsatellite markers for H. chillensis to assess the genetic variability and patterns of population structure of the species. •
METHODS AND RESULTS
Twelve microsatellite primers were isolated using a CT- and GT-enriched genomic library. PCR amplification detected one to five alleles, with 2.91 alleles per locus on average. Tested for cross-amplification, all primer pairs were successfully amplified in 10 South American species and in the putative ancestor of the group, H. angustifolia. •
CONCLUSIONS
The microsatellites can be used to assess genetic diversity and population structure of H. chillensis. Application in other species will focus on the elucidation of adaptive radiation of the genus in South America.
研究前提
Hypochaeris 被认为是一个生物模型，可用于了解南美洲维管植物的进化过程，特别是该大陆温带地区的进化过程。我们报告了为 H. chillensis 开发和表征微卫星标记的方法，以评估该物种的遗传变异和种群结构模式。
方法和结果
使用 CT 和 GT 富集基因组文库分离了 12 个微卫星引物。PCR 扩增检测到每个位点 1 到 5 个等位基因，平均每个位点有 2.91 个等位基因。在 10 种南美物种和该组假定的祖先 H. angustifolia 中，所有引物对均成功扩增。
结论
这些微卫星可用于评估 H. chillensis 的遗传多样性和种群结构。在其他物种中的应用将侧重于阐明南美洲该属的适应性辐射。
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