石松科多态性简单序列重复标记的开发
Development of polymorphic simple sequence repeat markers in (Lycopodiaceae).
作者信息
Guo Bin, Ren Jing-Yu, He Mei-Na, Yao Kai, Wang Tian-Shu, Wang Li-Qing, Liu Xin, He Wei, Fu Yan-Ping, Wang De-Li, Wei Ya-Hui
机构信息
Key Laboratory of Resource Biology and Biotechnology in Western China Department of Life Science Northwest University Xi'an 710069 People's Republic of China.
Hainan Branch Institute of Medicinal Plant Development Peking Union Medical College and Chinese Academy of Medical Sciences Haikou Hainan 570311 People's Republic of China.
出版信息
Appl Plant Sci. 2019 Jul 12;7(7):e11273. doi: 10.1002/aps3.11273. eCollection 2019 Jul.
PREMISE
The natural population size of (Lycopodiaceae) has dramatically decreased and the species has become endangered due to overexploitation. Here, we developed simple sequence repeat (SSR) markers for to survey both its genetic diversity and population structure.
METHODS AND RESULTS
Based on 177 individuals, 120 SSR primer pairs were developed and optimized from five regions of the transcriptomic data. Of these primer pairs, 20 were successfully amplified and 10 showed obvious polymorphism. These polymorphic loci were investigated to study the genetic diversity of . Two to 11 alleles per locus were identified, the level of observed heterozygosity ranged from 0.00 to 1.00, and the level of expected heterozygosity ranged from 0.19 to 0.79. All loci were successfully amplified in , , and .
CONCLUSIONS
The 10 polymorphic primer pairs developed here will be valuable for studies of the endangered and other related species.
前提
石松科某植物的自然种群数量已大幅减少，且由于过度开发该物种已濒临灭绝。在此，我们开发了简单序列重复（SSR）标记用于该植物，以调查其遗传多样性和种群结构。
方法与结果
基于177个个体，从该植物转录组数据的五个区域开发并优化了120对SSR引物对。在这些引物对中，20对成功扩增，10对表现出明显的多态性。对这些多态性位点进行研究以分析该植物的遗传多样性。每个位点鉴定出2至11个等位基因，观察到的杂合度水平在0.00至1.00之间，预期杂合度水平在0.19至0.79之间。所有位点在该植物的三个相关物种中均成功扩增。
结论
这里开发的10对多态性引物对将对濒危该植物及其他相关物种的研究具有重要价值。
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