为濒危孑遗针叶树水杉开发23个新型多态性EST-SSR标记。
Development of 23 novel polymorphic EST-SSR markers for the endangered relict conifer Metasequoia glyptostroboides.
作者信息
Jin Yuqing, Bi Quanxin, Guan Wenbin, Mao Jian-Feng
机构信息
National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, People's Republic of China.
School of Nature Conservation, Beijing Forestry University, Beijing 100083, People's Republic of China.
出版信息
Appl Plant Sci. 2015 Sep 11;3(9). doi: 10.3732/apps.1500038. eCollection 2015 Sep.
PREMISE OF THE STUDY
Metasequoia glyptostroboides is an endangered relict conifer species endemic to China. In this study, expressed sequence tag-simple sequence repeat (EST-SSR) markers were developed using transcriptome mining for future genetic and functional studies.
METHODS AND RESULTS
We collected 97,565 unigene sequences generated by 454 pyrosequencing. A bioinformatics analysis identified 2087 unique and putative microsatellites, from which 96 novel microsatellite markers were developed. Fifty-three of the 96 primer sets successfully amplified clear fragments of the expected sizes; 23 of those loci were polymorphic. The number of alleles per locus ranged from two to eight, with an average of three, and the observed and expected heterozygosity values ranged from 0 to 1.0 and 0.117 to 0.813, respectively.
CONCLUSIONS
These microsatellite loci will enrich the genetic resources to develop functional studies and conservation strategies for this endangered relict species.
研究前提
水杉是中国特有的濒危孑遗针叶树种。在本研究中，通过转录组挖掘开发了表达序列标签-简单序列重复（EST-SSR）标记，用于未来的遗传和功能研究。
方法与结果
我们收集了通过454焦磷酸测序产生的97,565条单基因序列。生物信息学分析鉴定出2087个独特的假定微卫星，从中开发了96个新型微卫星标记。96对引物中有53对成功扩增出预期大小的清晰片段；其中23个位点具有多态性。每个位点的等位基因数从2到8不等，平均为3个，观察到的杂合度值和预期杂合度值分别为0至1.0和0.117至0.813。
结论
这些微卫星位点将丰富遗传资源，以开展对这种濒危孑遗物种的功能研究和保护策略。
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