Yang Yan, Dai Liangfang, Wu Decai, Dong Limin, Tu Yisheng, Xie Jiankun, Luo Xiangdong
College of Life Science, Jiangxi Normal University, Nanchang 330022, China.
Plants (Basel). 2021 May 31;10(6):1112. doi: 10.3390/plants10061112.
is a traditional herb and endangered Chinese medicinal material, which has attracted much attention due to its production of Huperzine A (HupA). In vitro propagation of is considered a new way to relieve the resource pressure of . In this study, three different genotypic wild were used for in vitro propagation. Then, the antioxidant activity and the content of HupA in the regenerated were investigated. The results showed the survival rate of the explant was increased to 25.37% when using multiple sterilization processes. The best induction medium for was the Schenk and Hildebrandt (SH) medium supplemented with 0.5 mg·L Naphthalene acetic acid (NAA) and 0.1 mg·L 2,4-Dichlorophenoxyacetic acid (2,4-D), where the regeneration rate of the explant was to 57.04%. The best proliferation medium was the SH medium with NAA (1.0 mg·L), as the biomass of in vitro tissue increased 164.17 ± 0.41 times. High-performance liquid chromatography analysis showed that the in vitro culture of three genotypes could produce HupA and the content of HupA was 53.90-87.17 µg·g. The antioxidant experiment showed that the methanol extract of in vitro had higher antioxidant activity than that of wild . This study provides a reliable in vitro culture protocol and laid an important foundation for the antioxidant capacity of the thallus and the content of HupA.
是一种传统草药和濒危中药材，因其能产生石杉碱甲（HupA）而备受关注。的离体繁殖被认为是缓解资源压力的一种新途径。在本研究中，使用了三种不同基因型的野生进行离体繁殖。然后，研究了再生植株的抗氧化活性和石杉碱甲的含量。结果表明，采用多次灭菌处理时，外植体的成活率提高到了25.37%。的最佳诱导培养基是添加了0.5 mg·L萘乙酸（NAA）和0.1 mg·L 2,4-二氯苯氧乙酸（2,4-D）的 Schenk 和 Hildebrandt（SH）培养基，此时外植体的再生率达到了57.04%。最佳增殖培养基是添加NAA（1.0 mg·L）的SH培养基，离体组织的生物量增加了164.17±0.41倍。高效液相色谱分析表明，三种基因型的离体培养均可产生石杉碱甲，石杉碱甲的含量为53.90 - 87.17 µg·g。抗氧化实验表明，离体植株的甲醇提取物比野生植株具有更高的抗氧化活性。本研究提供了可靠的离体培养方案，为植株的抗氧化能力和石杉碱甲的含量奠定了重要基础。
