Contreras Verónica, Londoño Andrés F, Miranda Jorge, Mattar Salim, Acevedo-Gutiérrez Leidy Y, Diaz Francisco J, Rodas Juan D
Instituto de Investigaciones Biológicas del Trópico, Universidad de Córdoba, Colombia.
Grupo de Investigación en Ciencias Veterinarias, Centauro, Universidad de Antioquia, Colombia.
J Arthropod Borne Dis. 2019 Mar 30;13(1):1-8. eCollection 2019 Mar.
is an emergent Rickettsial agent whose main vector is , but ticks, mites and lice are also infected. We aimed to search for molecular evidence of spp. in fleas collected from dogs and wild rodents (Heteromys anomalous) from three villages of Córdoba and Antioquia provinces (Northern of Colombia), where outbreaks of rickettsioses have occurred, and discuss the possible role of fleas on endemic/enzootic regions for rickettsia.
During 2010 and 2012, 649 and 24 fleas were removed from dogs and wild rodents (), respectively, in 3 locations from Córdoba and Antioquia provinces (Colombia). These fleas were tested into pools for Rickettsial infection by PCR, targeting , and Rickettsial genes.
Almost 20% (30/153) of pools contained Rickettsial DNA. The fragments of gene showed high identity values between sequences from Necocli and Los Cordobas with strain from Senegal (100% and 99.7% respectively) and all were highly related by phylogenetic analyses. Rickettsial DNA in pools of was not detected.
Our findings highlighted the endemicity of the infection by in fleas from northern of Colombia and showed the likely importance of dogs as hosts of fleas and their potential role as reservoirs of
是一种新兴的立克次氏体病原体，其主要传播媒介是 ，但蜱、螨和虱子也会被感染。我们旨在寻找从哥伦比亚北部科尔多瓦省和安蒂奥基亚省的三个村庄的狗和野生啮齿动物（异鼠）身上采集的跳蚤中 属物种的分子证据，这些地区曾发生立克次氏体病疫情，并讨论跳蚤在立克次氏体地方性/动物地方性流行地区可能发挥的作用。
在2010年至2012年期间，分别从哥伦比亚科尔多瓦省和安蒂奥基亚省的3个地点的狗和野生啮齿动物（ ）身上采集了649只 和24只跳蚤。通过针对 、 和 立克次氏体基因的PCR检测这些跳蚤混合样本是否感染立克次氏体。
近20%（30/153）的 混合样本含有立克次氏体DNA。 基因片段在来自内科克利和洛斯科尔多瓦斯的序列与来自塞内加尔的 菌株之间显示出高同一性值（分别为100%和99.7%），并且通过系统发育分析所有序列都高度相关。在 混合样本中未检测到立克次氏体DNA。
我们的研究结果突出了哥伦比亚北部跳蚤中 感染的地方性，并表明狗作为 跳蚤宿主的可能重要性及其作为 的潜在储存宿主的作用
