智利狐蚤中巴尔通体属和猫立克次体的分子鉴定。
Molecular identification of Bartonella spp. and Rickettsia felis in fox fleas, Chile.
机构信息
Instituto Agroalimentario de Aragón-IA2 (Universidad de Zaragoza-CITA), Miguel Servet 177, 50013 Zaragoza, Spain; Fundación ARAID, Avda. de Ranillas, 50018 Zaragoza, Spain; Facultad de Ciencias de la Vida, Universidad Andres Bello, República 440, Santiago, Chile.
Instituto de Ciencias Clínicas Veterinarias, Facultad de Ciencias Veterinarias, Universidad Austral de Chile, Valdivia, Chile.
出版信息
Comp Immunol Microbiol Infect Dis. 2023 May;96:101983. doi: 10.1016/j.cimid.2023.101983. Epub 2023 Apr 20.
Seventy-five flea pools (one to ten fleas per pool) from 51 Andean foxes (Lycalopex culpaeus) and five South American grey foxes or chillas (Lycalopex griseus) from the Mediterranean region of Chile were analyzed for the presence of DNA of Bartonella spp. and Rickettsia spp. through quantitative real-time PCR for the nouG and gltA genes, respectively. Positive samples were further characterized by conventional PCR protocols, targeting gltA and ITS genes for Bartonella, and gltA, ompA, and ompB genes for Rickettsia. Bartonella was detected in 48 % of the Pulex irritans pools (B. rochalimae in three pools, B. berkhoffii in two pools, B. henselae in one pool), and 8 % of the Ctenocephalides felis felis pools (B. rochalimae, one pool). Rickettsia was confirmed in 11 % of P. irritans pools and 92 % of the Ct. felis pools. Characterization confirmed R. felis in all sequenced Rickettsia-positive pools. All Ct. canis pools were negative. A Ct. felis pool from a wild-found domestic ferret (Mustela putorius furo) also resulted positive for R. felis. Although opportunistic, this survey provides the first description of zoonotic pathogens naturally circulating in fleas parasitizing Chilean free-living carnivores.
从智利地中海地区的 51 只安第斯狐（Lycalopex culpaeus）和 5 只南美灰狐或 chillas（Lycalopex griseus）中采集了 75 个跳蚤池（每个池 1-10 只跳蚤），通过定量实时 PCR 分别针对 nouG 和 gltA 基因检测巴尔通体和立克次体属的 DNA。对阳性样本进一步采用常规 PCR 方案进行了特征分析，针对 Bartonella 的 gltA 和 ITS 基因，以及针对 Rickettsia 的 gltA、ompA 和 ompB 基因。在 48%的 P. irritans 池中检测到了 Bartonella（3 个池中为 B. rochalimae，2 个池中为 B. berkhoffii，1 个池中为 B. henselae），在 8%的 Ctenocephalides felis felis 池中检测到了 Bartonella（一个池中为 B. rochalimae）。在 11%的 P. irritans 池和 92%的 Ct. felis 池中证实了 Rickettsia。对所有测序的 Rickettsia 阳性池进行特征分析，均证实为 R. felis。所有 Ct. canis 池均为阴性。从一只野生的家养雪貂（Mustela putorius furo）中采集的 Ct. felis 池也对 R. felis 呈阳性。尽管机会性很强，但该调查首次描述了在寄生智利自由生活的食肉动物的跳蚤中自然循环的人畜共患病病原体。
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