Sugiharto Victor A, Widjaja Susana, Hartman Laurie J, Williams Maya, Myers Todd E, Simons Mark P
MSMR. 2019 Jul;26(7):18-23.
The Naval Infectious Diseases Diagnostic Laboratory (NIDDL) serves as a reference clinical laboratory that supports Department of Defense (DoD) military treatment facilities worldwide in the detection and identification of high-risk and emerging infectious diseases. Since the emergence of Zika virus (ZIKV) in the Western Hemisphere in 2016, the NIDDL has been a central hub for ZIKV testing for DoD personnel and beneficiaries. Samples collected during patients' clinical evaluations were screened for evidence of possible exposure to ZIKV using molecular and serological methods. An in-house ZIKV plaque reduction neutralization test was used to confirm the presence of ZIKV immunoglobulin M antibody. Of 1,420 individuals tested, ZIKV infection was confirmed by quantitative real-time polymerase chain reaction (PCR) assay in 11 (0.8%); an additional 26 recent flaviviral infections (possibly ZIKV) were identified based on serology (1.8%). These findings contribute to the understanding of the burden of ZIKV infections among DoD personnel and beneficiaries and highlight the role of the NIDDL in clinical diagnosis during emerging infectious disease outbreaks.
海军传染病诊断实验室（NIDDL）是一家参考临床实验室，为全球范围内的国防部（DoD）军事治疗机构提供支持，以检测和识别高风险及新出现的传染病。自2016年寨卡病毒（ZIKV）在西半球出现以来，NIDDL一直是为国防部人员及受益人群进行寨卡病毒检测的核心枢纽。在患者临床评估期间采集的样本，使用分子和血清学方法筛查是否有接触寨卡病毒的可能证据。采用内部寨卡病毒蚀斑减少中和试验来确认寨卡病毒免疫球蛋白M抗体的存在。在1420名接受检测的个体中，通过定量实时聚合酶链反应（PCR）检测确诊11例（0.8%）寨卡病毒感染；另外根据血清学鉴定出26例近期黄病毒感染（可能为寨卡病毒）（1.8%）。这些发现有助于了解国防部人员及受益人群中寨卡病毒感染的负担，并突出了NIDDL在新发传染病疫情期间临床诊断中的作用。
