Department of Plastic and Cosmetic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, P.R. China.
Guangdong Provincial Key Laboratory of Medical Biomechanics, Department of Anatomy, School of Basic Medical Science, Southern Medical University, Guangzhou, P.R. China.
Tissue Eng Part A. 2020 Jan;26(1-2):66-77. doi: 10.1089/ten.TEA.2019.0095. Epub 2019 Oct 24.
External volume expansion (EVE) is an effective method of adipose tissue regeneration. However, it remains unclear how EVE induces adipose tissue regeneration. In this study, we developed EVE devices to generate expanded prefabricated adipose tissue (EPAT) in rats and investigated cell proliferation, adipogenesis, and the expression of extracellular matrix (ECM) proteins during the 12 weeks suction. In addition, EPAT-generated decellularized adipose tissue (DAT) was used to assess the role of ECM proteins in cell proliferation and differentiation. Matrix deposition was significantly increased after EVE suction, with fibronectin and laminin showing the most dramatic changes. Fibronectin expression peaked during weeks 1-4, when Ki67 cells in EPAT peaked. Laminin expression peaked during weeks 8-12, when peroxisome proliferator-activated receptor-γ expression also peaked. , adipose-derived stem cells (ASCs) displayed a higher proliferation rate in week 1 DAT, when fibronectin expression was highest, whereas ASC adipogenesis was significantly higher on week 12 DAT, when laminin expression was abundant. These results showed that EVE device enhanced ECM deposition, which is closely related to cell proliferation and differentiation. Impact Statement Large soft tissue defects caused by cancer, trauma, or deformity remain a major challenge for reconstructive surgery. External volume expansion (EVE) successfully induces adipose tissue regeneration and shows great therapeutic potential in correction for soft tissue defect. This study showed that EVE enhanced the secretion of extracellular matrix (ECM) proteins and regulated ASC proliferation and differentiation through shifting matrix synthesis from fibronectin to laminin. These findings revealed the relation between ECM modulation and ASC behavior, indicating that EVE can induce adipose regeneration by regulating matrix synthesis.
外部容量扩张（EVE）是脂肪组织再生的有效方法。然而，EVE 如何诱导脂肪组织再生仍不清楚。在这项研究中，我们开发了 EVE 装置，以在大鼠中产生膨胀的预制脂肪组织（EPAT），并研究了在 12 周抽吸过程中的细胞增殖、脂肪生成和细胞外基质（ECM）蛋白的表达。此外，使用 EPAT 产生的去细胞脂肪组织（DAT）来评估 ECM 蛋白在细胞增殖和分化中的作用。EVE 抽吸后基质沉积显著增加，其中纤维连接蛋白和层粘连蛋白变化最为明显。纤维连接蛋白表达在第 1-4 周达到高峰，此时 EPAT 中的 Ki67 细胞达到高峰。层粘连蛋白表达在第 8-12 周达到高峰，此时过氧化物酶体增殖物激活受体-γ的表达也达到高峰。此外，脂肪来源干细胞（ASCs）在 DAT 的第 1 周表现出更高的增殖率，此时纤维连接蛋白表达最高，而 ASC 脂肪生成在 DAT 的第 12 周显著更高，此时层粘连蛋白表达丰富。这些结果表明，EVE 装置增强了 ECM 沉积，这与细胞增殖和分化密切相关。
